Circulating tumor cells (CTCs) usually shed from primary and metastatic tumors serve as an important tumor marker, and easily cause fatal distant metastasis in cancer patients. Accurately and effectively detecting CTCs in a peripheral blood sample is of great significance in early tumor diagnosis, efficacy evaluation, and postoperative condition monitoring. In this work, a TiO2@Ag nanostructure is structured as a SERS substrate, rhodamine 6G (R6G) is used as a Raman signal molecule, the reduced bovine serum protein (rBSA) acts as a protective agent, and folic acid (FA) acts as a target molecule to specifically recognize cancer cells. A TiO2@Ag-based SERS bioprobe is successfully prepared with the feature of ultrahigh sensitivity, good specificity, low toxicity, and high accuracy in CTC detection. The remarkable SERS activity of the TiO2@Ag nanostructure is synergistically contributed by surface plasmon resonance and photon-induced charge transfer mechanism. The limit of detection for rhodamine 6G (R6G) molecules adsorbed on the TiO2@Ag SERS substrate is 5 × 10-14 M, and the corresponding SERS enhancement factor can reach 7.61 × 107. The designed TiO2@Ag-R6G-rBSA-FA SERS bioprobe is effectively utilized in detecting various cancer cells in rabbit blood, and the limit of detection (LOD) for the target cancer cell is 1 cell per mL. Notably, CTCs in peripheral blood of six clinical liver cancer patients are successfully recognized via the TiO2@Ag-based SERS bioprobe. Accurately recognizing CTCs in peripheral blood based on the TiO2@Ag-R6G-rBSA-FA SERS bioprobe is also carefully verified by in situ immunofluorescence staining experiments, which directly supports the CTC detection accuracy of the SERS strategy. These results demonstrate that the TiO2@Ag-based SERS bioprobe has great application potential in early screening and diagnosis of tumors.A fundamental quantitative study of the vibrational resonances of three H216O H,D-isotopologues with a quartic Watson Hamiltonian was carried out using the resummation of the high-order (∼λn, n ≤ 203) divergent Rayleigh-Schrödinger perturbation theory (RSPT) series by quartic Padé-Hermite multivalued diagonal approximants PH[m,m,m,m,m], m ≤ 40. The resonance condition between a pair of states is formulated as the existence of a common complex energy solution branch point inside the unit circle |E(λj)|,  Re(λj)2 + Im(λj)2 ≤ 1. For the matrix formulation of the vibrational problem (VCI), the existence of common branch points is governed by the Katz theorem and they can be found as roots of discriminant polynomials. The main branches of the Padé-Hermite approximants typically reproduce VCI energies with high accuracy while alternative branches often fit nearby resonant states. The resummation of the RSPT series for H2O and D2O (up to the tenth polyad) revealed not only Fermi and Darling-Dennison resonances, but also unusual (0,2,-5) and (5,-2,0) resonance effects matching the (5,2,5) polyad structure, while the (3,2,1) structure was rigorously confirmed for HDO. It is demonstrated that the (5,2,5) polyad structure ensures good organization of high-energy resonating states and breaks down the classic (2,1,2) structure. The advocated methodology of quantitative description of resonance phenomena and revealing polyad structures is suitable for larger molecules and can be adapted to linear molecules and symmetric tops. Its application ensures rigorous classification of vibrational states and can be used in quantitative vibration-rotation spectroscopy.The competitive aggregated adsorption and molecular interactions between paclitaxel (PX) and mitomycin C (MMC) molecules on the surface of boron nitride nanosheets (BNNSs) were investigated using a molecular dynamics method. The potential capability of BNNSs to immobilize PX and MMC molecules was examined in detail. Structural parameters such as the radius of gyration of the drugs on the considered surface were calculated. The results indicate rapid and efficient adsorption of PX and MMC ligands onto BNNSs. The electrostatic contribution confirms the efficient self-aggregation of each drug onto the BNNS surface during the adsorption process in 100 ns simulation trajectories. The radial distribution function and dipole moments of water molecules have been considered to estimate the effect of water molecules on the adsorption of PX and MMC ligands onto BNNSs. The aggregation of MMC molecules onto BNNSs does not affect the aggregated adsorption of PX molecules. The maximum values of interaction energies and hydrogen bonds for PX molecules indicate that PX molecules overtake MMC molecules via competitive aggregation. The efficient and favorable delivery capability of boron nitride nanosheets to adsorb and deliver self-aggregated PX and MMC molecules has been revealed by molecular dynamics simulation results.The introduction of organic functionalities into porous inorganic materials not only makes the resulting hybrid porous framework to be more flexible and hydrophobic, but also provides additional scope for anchoring metal binding sites, which is beneficial for different frontline applications. Furthermore, the nanoscale porosity and high surface area of these organic-inorganic hybrid materials offer a better dispersion of active sites, which greatly enhances their application potential in adsorption, sensing, drug-delivery, energy storage, optoelectronics, light harvesting and catalysis. Easy post-synthetic functionalization of these hybrid materials has widened their application potential. Herein, we highlight several important synthetic strategies to design a wide range of organic-inorganic hybrid porous materials starting from the respective molecular precursors and their task-specific applications in energy and environmental research. We also outline the recent developments in their respective application areas together with various challenges that need to be overcome.For the first time, a novel class of deep-blue (DB)-emitting Csx(NH4)1-xPbCl2Br (0.3 ≤ x ≤ 1) perovskite nanocrystals (PNCs) were prepared by a facile ligand-assisted one-step ball milling method. The resulted PNCs are characterized by high chlorine content (66.7%) and excellent color purity. Their photoluminescence position can be finely modulated from 434 nm to 447 nm, which extends notably beyond the current Rec. 2020 color standard, by the NH4+ content. Among them, Cs0.3(NH4)0.7PbCl2Br shows the highest quantum yield close to 40%. The PNCs exhibit high phase and optical stability under ambient conditions and UV light according to the NH4+ content. This work offers a new avenue to produce DB perovskites for future full-color displays and optoelectronics.

To determine and compare the accuracy and reliability of shade matching using the conventional and smartphone virtual methods.

A phantom head with both maxillary central incisors removed was set up. A central incisor of various standard shades was inserted into the phantom head. Five undergraduate and five postgraduate students were asked to select the closest shade to match the central incisor using the Vita Classic shade guide. The procedure was then repeated using images taken by a smartphone. Each technique was repeated three times. Differences in accuracy of shade matching between the two techniques for every shade tab and between undergraduate and postgraduate dental students were compared using chi-square statistical analysis. The P value was set at .001. Differences in intra-rater and inter-rater reliability between the two techniques and among the three sessions were compared using paired t test and analysis of variance (ANOVA), respectively, with a P value of .05. The reliability of both techniques was further measured using Cohen kappa statistical test.

The smartphone virtual shade-matching technique showed significantly higher accuracy in shade matching with most of the tested shade tabs than the conventional method (P < .001), irrespective of observers’ clinical experience. Higher repeatability was found in the virtual technique than the conventional technique, with higher intra-rater and inter-rater reliability observed.

Smartphone virtual shade matching showed better accuracy and reliability than the conventional method and could be used as an alternative shade-matching method.

Smartphone virtual shade matching showed better accuracy and reliability than the conventional method and could be used as an alternative shade-matching method.

To compare wear behavior, durability during in vitro mastication simulation, and fracture force of an established and a novel lithium disilicate CAD/CAM material, as well as to examine the impact of cementation and reduced ceramic thickness on durability and fracture force.

Specimens (n = 8 per group) were prepared from lithium disilicate (LS

; IPS e.max, Ivoclar Vivadent) and advanced lithium disilicate (ALD; Cerec Tessera, Dentsply Sirona). Specimens were polished, and two-body wear test and thermocycling were performed (50 N, 120,000 cycles, 1.6 Hz, H

O dist., 5°C/55°C, 600 cycles). Maximum vertical loss, surface roughness, surface roughness depth, and antagonist wear were determined. Single crowns (n = 8 per group; thickness 1.5 mm/1.0 mm) were manufactured from LS

and ALD and mounted on human molar teeth with adhesive resin (AB; CalibraCeram, Dentsply Sirona), glass-ionomer cement (GIC; Ketac Cem, 3M ESPE), and hybrid glass-ionomer cement (HGIC; Calibra Bio, Dentsply Sirona). Thermocycling and mechanical loading (2 × 3000 × 5°C/55°C, 2 minutes, H

0 dist., 1.2 × 10

50 N) were performed. Fracture force was determined by a universal testing machine (1446, ZwickRoell), and one-way analysis and Bonferroni post hoc test (α = .05) were used for statistical analyses.

Mean (ALD 210 ± 42.4 μm; LS

264.3 ± 56.1 μm) and maximum (ALD 391.1 ± 86.3 μm; LS

518.3 ± 113.2 μm) wear between groups were significantly different (P ≤ .047). Fracture force varied between 1,911.4 ± 468.4 N (ALD/AB 1 mm) and 2,995.3 ± 880.6 N (LS

/GIC), without significant differences (P ≥ .152).

ALD showed better wear behavior than LS

, but provided similar fracture force. Cementation and reduction of ceramic thickness had only minor effects on fracture force.

ALD showed better wear behavior than LS2, but provided similar fracture force. Cementation and reduction of ceramic thickness had only minor effects on fracture force.

To test a newly introduced implant-abutment material combination against bacterial endotoxin leakage in a human whole blood assay.

Two dental implant systems with internal connections and the following material combinations at the implant-abutment interface (IAI) were used (implant material/abutment material) yttrium-stabilized tetragonal zirconium dioxide (Y-TZP)/polyetherketoneketone (PEKK), and titanium (Ti/Ti). Test implants were inoculated with lipopolysaccharide (LPS) and sealed and submerged in human whole blood. Untreated implants served as the control groups. Changes in gene expression levels of inflammatory markers indicating LPS leakage were assessed after 1, 8, and 24 hours using quantitative real-time polymerase chain reaction.

In the Y-TZP/PEKK test group, a significant influence of the implant system (P < .001) on increases in gene expression indicating leakage were detected after 8 hours for TLR-4 and after 24 hours for interleukin 1-β and nuclear factor kappa-light-chain-enhancer of activated B-cells (NF-κB), indicating microleakage of LPS at the IAI.