gambiae. First instar larvae of each group from the crossing (here after An. coluzzii, Hybrid COL/GAM, Hybrid GAM/COL and An. gambiae, respectively) were transplanted in a field experiment with predation effect. Emergence success, development time of larvae and body size of the newly emerging adults were estimated as fitness components and then compared between parental species and F1 hybrids in absence and in presence of predators. Our findings confirm that An. coluzzii had higher fitness than An. gambiae in presence of predators versus in absence of predators. Moreover, the fitness of the F1 hybrid COL/GAM whose female parent was An. coluzzii matched that of An. coluzzii while that of the F1 reciprocal hybrid GAM/COL was similar to An. gambiae.Epigenetic factors such as DNA methylation act as mediators in the interaction between genome and environment. Variation in the epigenome can both affect phenotype and be inherited, and epigenetics has been suggested to be an important factor in the evolutionary process. During domestication, dogs have evolved an unprecedented between-breed variation in morphology and behavior in an evolutionary short period. In the present study, we explore DNA methylation differences in brain, the most relevant tissue with respect to behavior, between wolf and dog breeds. We optimized a combined method of genotype-by-sequencing (GBS) and methylated DNA immunoprecipitation (MeDIP) for its application in canines. Genomic DNA from the frontal cortex of 38 dogs of 8 breeds and three wolves was used. GBS and GBS-MeDIP libraries were prepared and sequenced on Illuma HiSeq2500 platform. The reduced sample represented 1.18 ± 0.4% of the total dog genome (2,4 billion BP), while the GBS-MeDIP covered 11,250,788 ± 4,042,106 unique base pairs. We find substantial DNA methylation differences between wolf and dog and between the dog breeds. The methylation profiles of the different groups imply that epigenetic factors may have been important in the speciation from dog to wolf, but also in the divergence of different dog breeds. Specifically, we highlight methylation differences in genes related to behavior and morphology. We hypothesize that these differences are involved in the phenotypic variation found among dogs, whereas future studies will have to find the specific mechanisms. Our results not only add an intriguing new dimension to dog breeding but are also useful to further understanding of epigenetic involvement.Anthropogenic noise is an often-overlooked byproduct of urbanization and affects the soundscape in which birds communicate. Previous studies assessing the impact of traffic noise have focused on bird song, with many studies demonstrating the ability of birds to raise song frequency in the presence of low-frequency traffic noise to avoid masking. Less is known about the impact of traffic noise on avian alarm calls, which is surprising given the degree to which predator information within alarm calls may impact fitness. The objective of this study was to assess the impacts of traffic noise on the Black-capped Chickadees (Poecile atricapillus), a small non-migratory songbird with a well-studied and information-rich alarm call. We studied birds at eight locations in Stark County, Ohio, from 15 January to 7 March 2016, and used a taxidermic mount of an Eastern Screech-Owl to elicit alarm calls. In half of the trials, a pre-recorded traffic noise track was also broadcasted at 50 decibels. In noise trials, chickadee calls contained more introductory notes (P less then 0.001), more total notes (P less then 0.001), were of longer duration (P less then 0.001), and had lower introductory and D-note peak frequencies (P = 0.032 and P = 0.041, respectively). No differences were noted in the number of D-notes per call between noise and control trials. Modifying alarm call duration and frequency, without changing the number of D-notes, may be a strategy that chickadees use to convey predator information and to coordinate a threat-appropriate mobbing response when it is not possible to change call type. Our results add to the small, but growing, literature documenting the effects of anthropogenic noise on avian alarm calls, demonstrate the flexibility and complexity of chickadee calls given in response to predators, and may partially explain why chickadees adapt well to urban areas.

Chagas disease (CD) is highly endemic in the Bolivian Chaco. The municipality of Monteagudo has been targeted by national interventions as well as by Médecins Sans Frontières to reduce infection rates, and to decentralize early diagnosis and treatment. This study seeks to determine the knowledge and attitudes of a population with increased awareness and to identify remaining factors and barriers for sustained vector control, health care seeking behaviour, and access, in order to improve future interventions.

A cross-sectional survey was conducted among approximately 10% (n = 669) of the municipality of Monteagudo’s households that were randomly selected. Additionally, a total of 14 in-depth interviews and 2 focus group discussions were conducted with patients and key informants. Several attitudes and practices were identified that could undermine effective control against (re-)infection. Knowledge of clinical symptoms and secondary prevention was limited, and revealed specific misconceptions. Although 76%raditional and animal health sectors might help to overcome current barriers and advocate for patients’ rights.

Large scale interventions should be accompanied by operational research in order to identify misconceptions and unintended consequences early on, to generate accessible data for future interventions, and for rigorous evaluation. An integrated, community-based approach tackling social determinants and including both traditional and animal health sectors might help to overcome current barriers and advocate for patients’ rights.Studies of viral suppression on first-line antiretroviral therapy (ART) in persons living with human immunodeficiency virus (PLHIV) in Haiti are limited, particularly among PLHIV outside of the Ouest department, where the capital Port-au-Prince is located. This study described the prevalence and risk factors for delayed viral suppression among PLHIV in all geographic departments of Haiti between 2013 and 2017. Individuals who received viral load testing 3 to 12 months after ART initiation were included. Data on demographics and clinical care were obtained from the Haitian Active Longitudinal Tracking of HIV database. Multivariable logistic regression was performed to predict delayed viral suppression, defined as a viral load ≥1000 HIV-1 RNA copies/mL after at least 3 months on ART. Viral load test results were available for 3,368 PLHIV newly-initiated on ART. Prevalence of delayed viral suppression was 40%, which is slightly higher than previous estimates in Haiti. In the multivariable analysis, delayed viral suppression was significantly associated with younger age, receiving of care in the Ouest department, treatment with lamivudine (3TC), zidovudine (AZT), and nevirapine (NVP) combined ART regimen, and CD4 counts below 200 cells/mm3. In conclusion, this study was the first to describe and compare differences in delayed viral suppression among PLHIV by geographic department in Haiti. We identified populations to whom public health interventions, such as more frequent viral load testing, drug resistance testing, and ART adherence counseling should be targeted.Nitrogen (N) is a key element for the production of potato. The N uptake efficiency, N use efficiency and increased N utilization efficiency can be decreased by N deficiency treatment. We performed this study to investigate the association between transcriptomic profiles and the efficiencies of N in potato. Potato cultivars „Yanshu 4” (short for Y), „Xiabodi” (cv. Shepody, short for X) and „Chunshu 4” (short for C) were treated with sufficient N fertilizer and deficient N fertilizer. Then, the growth parameters and tuber yield were recorded; the contents of soluble sugar and protein were measured; and the activities of enzymes were detected. Leaf and root transcriptomes were analyzed and differentially expressed genes (DEGs) in response to N deficiency were identified. The results showed that N deficiency decreased the nitrate reductase (NR), glutamine synthetase (GS) and root activity. Most of the DEGs between N-treated and N-deficiency participate the processes of transport, nitrate transport, nitrogen compound transport and N metabolism in C and Y, not in X, indicating the cultivar-dependent response to N deficiency. DEGs like glutamate dehydrogenase (StGDH), glutamine synthetase (StGS) and carbonic anhydrase (StCA) play key roles in these processes mentioned above. DEGs related to N metabolism showed a close relationship with the N utilization efficiency (UTE), but not with N use efficiency (NUE). The Major Facilitator Superfamily (MFS) members, like nitrate transporter 2.4 (StNRT2.4), 2.5 (StNRT2.5) and 2.7 (StNRT2.7), were mainly enriched in the processes associated with response to stresses and defense, indicating that N deficiency induced stresses in all cultivars.Physical distancing, as a measure to contain the spreading of Covid-19, is defining a „new normal”. Unless belonging to a family, pedestrians in shared spaces are asked to observe a minimal (country-dependent) pairwise distance. Coherently, managers of public spaces may be tasked with the enforcement or monitoring of this constraint. As privacy-respectful real-time tracking of pedestrian dynamics in public spaces is a growing reality, it is natural to leverage on these tools to analyze the adherence to physical distancing and compare the effectiveness of crowd management measurements. Typical questions are „in which conditions non-family members infringed social distancing?”, „Are there repeated offenders?”, and „How are new crowd management measures performing?”. Notably, dealing with large crowds, e.g. in train stations, gets rapidly computationally challenging. In this work we have a two-fold aim first, we propose an efficient and scalable analysis framework to process, offline or in real-time, pedestrian tracking data via a sparse graph. The framework tackles efficiently all the questions mentioned above, representing pedestrian-pedestrian interactions via vector-weighted graph connections. On this basis, we can disentangle distance offenders and family members in a privacy-compliant way. Second, we present a thorough analysis of mutual distances and exposure-times in a Dutch train platform, comparing pre-Covid and current data via physics observables as Radial Distribution Functions. The versatility and simplicity of this approach, developed to analyze crowd management measures in public transport facilities, enable to tackle issues beyond physical distancing, for instance the privacy-respectful detection of groups and the analysis of their motion patterns.Alterations in autonomic activity are well established in fibromyalgia syndrome (FMS). Previous studies found reduced parasympathetic activity and sympathetic reactivity to physical and stress manipulations. However, sympathetic activity at rest has not been well studied in FMS. Sweating is exclusively controlled by sympathetic mechanisms. In this study, skin conductance (SC), as an indirect measure of sweating, was analyzed in 45 women with FMS and 38 healthy women. Tonic SC levels were recorded during a 4-minute rest period, and a breathing maneuver consisting of deep breathing with posterior breath holding was used to evoke SC responses. Associations of tonic SC with state anxiety and body temperature, measured in the hand, were explored to determine sweat functionality. The results showed reduced tonic SC levels, with a less marked decrease in SC during the recording period, and blunted SC reactivity to the breathing manipulation in FMS patients relative to healthy participants. Positive associations of SC with state anxiety and body temperature were observed in healthy participants, but these associations were absent in FMS patients.