Although challenging, our approach appears safe and feasible and may help others to set up or optimize their procedures for cancer treatment or for other exceedingly vulnerable patient cohorts.

Parkinson’s disease (PD) progressively impairs motor and cognitive performance. The current tools to detect decline in motor and cognitive functioning are often impractical for busy clinics and home settings. To address the gap, we designed an instrumented trail-making task (iTMT) based on a wearable sensor (worn on the shin) with interactive game-based software installed on a tablet. The iTMT test includes reaching to 5 indexed circles, a combination of numbers (1-3) and letters (A&B) randomly positioned inside target circles, in a sequential order, which virtually appears on a screen kept in front of the participants, by rotating one’s ankle joint while standing and holding a chair for safety. By measuring time to complete iTMT task (iTMT time), iTMT enables quantifying cognitive-motor performance.

This study’s objective is to examine the feasibility of iTMT to detect early cognitive-motor decline in PDs.

Three groups of volunteers, including 14 cognitively normal (CN) older adults, 14 PDs, anibility and early results supporting the potential application of iTMT to determine cognitive-motor and distinguishing individuals with MCI and PD from CN-older adults. Future studies are warranted to test the ability of iTMT to track its subtle changes over time.An adverse maternal environment (AME) predisposes adult offspring toward cognitive impairment in humans and mice. However, the underlying mechanisms remain poorly understood. Epigenetic changes in response to environmental exposure may be critical drivers of this change. Epigenetic regulators, including microRNAs, have been shown to affect cognitive function by altering hippocampal neurogenesis which is regulated in part by brain-derived neurotropic factor (BDNF). We sought to investigate the effects of AME on miR profile and their epigenetic characteristics, as well as neurogenesis and BDNF expression in mouse hippocampus. Using our mouse model of AME which is composed of maternal Western diet and prenatal environmental stress, we found that AME significantly increased hippocampal miR-10b-5p levels. We also found that AME significantly decreased DNA methylation and increased accumulations of active histone marks H3 lysine (K) 4me3, H3K14ac, and -H3K36me3 at miR-10b promoter. Furthermore, AME significantly decreased hippocampal neurogenesis by decreasing cell numbers of Ki67+ (proliferation marker), NeuroD1+ (neuronal differentiation marker), and NeuN+ (mature neuronal marker) in the dentate gyrus (DG) region concurrently with decreased hippocampal BDNF protein levels. We speculate that the changes in epigenetic profile at miR-10b promoter may contribute to upregulation of miR-10b-5p and subsequently lead to decreased BDNF levels in a model of impaired offspring hippocampal neurogenesis and cognition in mice.This work is intended to study the radioprotective effect of palladium α-lipoic acid nano-complex (PLAC) on hemoglobin molecule in vitro. Blood samples were obtained from adult male rats weighing 120-150 g after dissection, using heparinized needles. Each blood sample was divided into four groups; the first group was kept untreated as control, palladium α-lipoic acid (PLAC) was added to the second group at concentration 2% v/v, the third group was exposed to 100 Gy gamma radiation and the forth group was irradiated with the addition of PLAC. Hemoglobin was extracted and prepared for measurement. The effects on the hemoglobin molecule were evaluated by FTIR and UV-visible spectroscopy. The results showed that PLAC increases the optical energy gap of the transition of the amino acid side chains and affects the spatial distribution of the globin part. Gamma radiation affects mainly the globin part, results in unfolding of the protein structure and perturbation in the relative orientation of the transition dipole moments. Addition of PLAC to the blood samples prior to irradiation was shown to provide protective effects which can be attributed to its ability to neutralize the free radicals.Nondestructive instrumental identification of the green tea quality instead of professional human panel tests is highly desired for industrial application recently. The special flavor is a key quality-trait that influence consumer preference. However, flavonoids, as well as sensory-associated compounds, which play a critical role in the quality-traits profile of green tea samples have been poorly investigated. In this study, we were proposing an objective and accurate near infrared spectroscopy (NIRS) profile to support quality control within the entire green tea sensory evaluation chain, the complexity of green tea samples’ sensory analysis was performed by two complementary methods the standard calculation and the novel NIRS roadmap coupled with chemometrics. The green tea samples’ physical quality, gustatory index, and nutritional index were measured respectively, which taking into consideration the gustatory evaluation of green tea for five commercially representative overall quality („very bad”, „bad”, ” However, the established models should be improved by more green tea samples from different regions.Hydrazine and mercury (Hg) poisoning represented a serious hazard to human health. So, developing method to detect and recognize them is highly desirable. Here, we prepared a multifunctional colorimetric and fluorescent probe (PI-Rh) consisting of a phenanthroimidazole (PI) dye conjugated with a Rhodamine (Rh) group for the effective recognition of hydrazine and Hg2+, induvidually and collectively, with different colorimetric and fluorescence outputs. Probe PI-Rh displays low detection limits measured to be 0.0632 μM (~2 ppb) and 0.0101 μM (~2 ppb) respectively for hydrazine and Hg2+ with high selectivity and excellent sensitivity. Moreover, the experimental results indicated that the superiority of this probe lied in its wide applications, for example, successful response in real water, and soil analysis. Interestingly, an visual, rapid, and real-time detection of gaseous hydrazine can be realized with 0.2793 μM detection limit using the facile PI-Rh-impregnated test paper.The content of insensitive agent is an important parameter that has been shown correlated with the combustion characteristic of double-base oblate spherical propellant (DOSP). This work focused on the feasibility of simultaneous monitoring the content of insensitive agent (dibutyl phthalate (DBP) and N, N’-dimethyl-N, N’-diphenylurea (C2)) in DOSP by using near-infrared (NIR) spectroscope coupled with partial least squares (PLS). The optimal spectral intervals for creating models of DBP and C2 corresponded to 5964 cm-1-4212 cm-1 and 6240 cm-1-4380 cm-1, respectively. It had been demonstrated that derivative tools were more suitable for spectral preprocessing as which had the lowest root mean squares error of cross-validation (RMSECV). The best-performance models of DBP and C2 were built under 4 and 7 PLS factors, respectively. The results showed that the determination coefficients of calibration (Rc2) and the root mean squares error of calibration (RMSEC) were 0.9771 and 0.0173 for DBP; 0.984 and 0.0072 for C2, respectively. Besides, the developed models exhibited excellent ability in prediction with the determination coefficients in prediction (RP2) and the root mean squares error in prediction (RMSEP) of 0.9681 and 0.0275 for DBP, and of 0.9554 and 0.0107 for C2, respectively. The residual predictive deviation (RPD) of prediction set were 5.68 and 5.12 for DBP and C2, respectively. The average relative errors of the proposed and reference methods were 0.652% for DBP, and 0.429% for C2, revealing a good correlation between the reference values and predicted values. Therefore, it concluded that the proposed plan has shown to be an attractive means since its efficient and highly accurate which could provide a better option for quality control in the large-scale production of DOSP.Dermal fibroblasts are a promising candidate for cellular-based therapies for thermal wound healing because of their capacity of producing extracellular matrix (ECM), promoting wound contraction and the synthesis of type I collagen, and secreting growth factors. miRNAs (MicroRNAs) might mediate the role of TGF-β1(Transforming Growth Factor-beta 1), one of the major profibrotic cytokines, in improving thermal injury repair. In the present study, we observed the abnormal downregulation of TGF-β1 following thermal injury in the burnt dermis (in vivo) and heat-stimulated human dermal fibroblasts (in vitro). TGF-β1 overexpression reversed heat stimulation-induced repression on fibroblast viability, migration, and ECM synthesis. As demonstrated by online tool prediction and experimental analysis, miR-506-3p downregulated TGF-β1 levels via directly targeting TGFB1. In heat-stimulated human dermal fibroblasts, miR-506-3p expression showed to be significantly upregulated. miR-506-3p inhibition also reversed heat stimulation-induced repression on fibroblast viability, migration, and ECM synthesis; more importantly, TGF-β1 silencing aggravated the thermal injury in vitro and significantly reversed the effects of miR-506-3p inhibition on heat-stimulated dermal fibroblasts. In conclusion, miR-506-3p and its downstream target TGF-β1 form a regulatory axis, modulating the cell viability, migration, and ECM synthesis in human dermal fibroblasts following burn injury.Exercise during pregnancy has been shown to be associated with improved health outcomes both during and after pregnancy for mother and fetus across the lifespan. Increasing physical activity and reducing sedentary behaviour during pregnancy have been recommended by many researchers and clinicians-alike. It is thought that the placenta plays a central role in mediating any positive or negative pregnancy outcomes. The positive outcomes obtained through prenatal exercise are postulated to result from exercise-induced regulation of maternal physiology and placental development. Considerable research has been performed to understand the placenta’s role in pregnancy-related diseases, such as preeclampsia, fetal growth restriction, and gestational diabetes mellitus. However, little research has examined the potential for healthy lifestyle and behavioural changes to improve placental growth, development, and function. While the placenta represents the critical maternal-fetal interface responsible for all gas, nutrient, and waste exchange between the mother and fetus, the impact of exercise during pregnancy on placental biology and function is not well known. This review will focus on prenatal exercise and its promising influence on the structures of the maternal-fetal interface, with particular emphasis on the placenta. Potential molecular mechanistic hypotheses are presented to aid future investigations of prenatal exercise and placental health.Anoctamin 7 (ANO7) is a member of the transmembrane protein TMEM16 family. It has a conservative topology similar to other members in this family, such as the typical eight-transmembrane domain, but it also has unique features. Although the ion channel role of ANO7 has been well accepted, evolutionary analyses and relevant studies suggest that ANO7 may be a multi-facet protein in function. Studies have shown that ANO7 may also function as a scramblase. ANO7 is highly expressed in prostate cancer as well as normal prostate tissues. A considerable amount of evidence has confirmed that ANO7 is associated with human physiology and pathology, particularly with the development of prostate cancer, which makes ANO7 a good candidate as a diagnostic and prognostic biomarker. In addition, ANO7 may be a potential target for prostate cancer immunotherapy. Antibody-based or T cell-mediated immunotherapies against prostate cancer by targeting ANO7 have been highly anticipated. ANO7 may also correlate with several other types of cancers or diseases, where further studies are warranted.