Anthropogenic input of sulfate (SO42-) in reservoirs may enhance bacterial sulfate reduction (BSR) under seasonally hypoxic conditions in the water column. However, factors that control BSR and its coupling to organic carbon (OC) mineralization in seasonally hypoxic reservoirs remain unclear. The present study elucidates the coupling processes by analyzing the concentrations and isotopic composition of dissolved inorganic carbon (DIC) and sulfur (SO42-, sulfide) species, and the microbial community in water of the Aha reservoir, SW China, which has high SO42- concentration due to the inputs from acid mine drainage about twenty years ago. The water column at two sites in July and October revealed significant thermal stratification. In the hypoxic bottom water, the δ13C-DIC decreased while the δ34S-SO42- increased, implying organic carbon mineralization due to BSR. The magnitude of S isotope fractionation (Δ34S, obtained from δ34Ssulfate-δ34Ssulfide) during the process of BSR fell in the range of 3.4‰ to 27.0‰ in July and 21.6‰ to 31.8‰ in October, suggesting a change in the community of sulfate-reducing bacteria (SRB). The relatively low water column stability in October compared to that in July weakened the difference of water chemistry and ultimately affected the SRB diversity. The production of DIC (ΔDIC) scaled a strong positive relationship with the Δ34S in July (p less then 0.01), indicating that high OC availability favored the survival of incomplete oxidizers of SRB. However, in October, Δ13C-DIC was correlated with the Δ34S in the bottom hypoxic water (p less then 0.01), implying that newly degraded OC depleted in 13C could favor the dominance of complete oxidizers of SRB which caused greater S isotope fractionation. Moreover, the sulfide supplied by BSR might stimulate the reductive dissolution of Fe and Mn oxides (Fe(O)OH and MnO2). The present study helps to understand the coupling of C and S in seasonally hypoxic reservoirs characterized by high SO42- concentration.Anaerobic fungi (Neocallimastigomycota) are promising lignocellulose-degrading microorganisms that can be exploited by the biofuel industry. While natural production of ethanol by these microorganisms is very low, there is a greater potential for their use in the biogas industry. More specifically, anaerobic fungi can contribute to biogas production by either releasing holocellulose or reducing sugars from lignocelluloses that can be used as a substrate by bacteria and methanogens involved in the anaerobic digestion (AD) process or by metabolizing acetate and formate that can be directly consumed by methanogens. Despite their great potential, the appropriate tools for engineering anaerobic fungi have not been established yet. The first section of this review justifies how the biofuel industry can benefit from using anaerobic fungi and is followed by their taxonomy. In the third section, the possibility of using anaerobic fungi for the consolidated production of bioethanol is briefly discussed. Nevertheless, ttly improve anaerobic fungi viability throughout the AD process.This study proposes a methodological framework to evaluate and rank climate models based on extreme climate indices of precipitation and temperature for impact studies in seven sectors Cryosphere, Energy, Forestry/GHGs, Health, Agriculture & Food Security, Disaster Risk Reduction (flood and drought), and Water Resources & Hydrology. The ranking of the climate models is based on their performance in sector-relevant extreme climate indices. Extreme climate indices for observed and climate models’ datasets for a historical period and overall performance statistics were used to create a payoff matrix. The payoff matrix then served as an input to a multi-criteria decision-making process to rank the climate models for each of the climate indices. The final sector-specific ranking was achieved by averaging the ranks obtained in the sector-relevant indices. The developed methodology is demonstrated with an application to the Songkhram River Basin (Thailand), a sub-basin of the Mekong. Eighteen CMIP6 GCMs are used for the proposed evaluation and ranking processes and four performance statistics were used. Weights to each of the four performance statistics were determined using the entropy method. Compromise programming was applied to rank the GCMs based on the distance technique. The results indicate that the six best performing models are different for different sectors, with the GFDL_CM4 model common in all the seven sectors considered in the study. KACE1_0_G, GFDL_ESM4, GFDL_CM4, MRI_ESM2_0, and ACCESS_ESM1_5 models are the five top (ranked 1 to 5 respectively) performing models for the Water Resources & Hydrology sector. The developed framework is generic and can be applied to any region or basin; at the same time, it can also provide researchers and policymakers with specific information on best-performing models for particular sectors.Anaerobic granular sludge (AnGS) was cultured to treat high-strength perchlorate (reaching to 4800 mg/L) wastewater by an anaerobic baffled reactor (ABR) system with five equal-volume compartments (C1-C5 compartments). Inoculated sludge completely granulated on day 104 with granule size of 0.50-0.75 mm and perchlorate removal efficiency reaching to 97% (influent perchlorate of 2000-4800 mg/L). The Cyclic voltammetry (CV) capacitance increased from 487.5, 465.8 and 407.8 μF to 576.5, 552.4, 549.6 μF in C1, C3 and C5 compartments of ABR system, respectively, suggesting the electron transfer capacity was enhanced under high-strength perchlorate stress. Meanwhile, adenosine triphosphate (ATP) value and electron transport system activity (ETSA) increased to 25.05, 22.87, 20.43 and 6.22, 4.87, 3.95 of C1, C3 and C5 compartments, respectively. The results suggested that high-strength perchlorate stress improved the microbial metabolic activity, which promoted secretion of extracellular polymeric substances (EPS). The more EPS could facilitate the formation and stability of AnGS under high-strength perchlorate stress. In addition, more reasonable metabolic division of labor in functional bacterial (Thauera and Comamonas) was beneficial to AnGS formation, which achieved high-strength perchlorate efficient removal. Finally, a positive feedback mechanism between AnGS formation and high-strength perchlorate removal was established through EPS, microbial metabolic activity and electron transfer characteristic in ABR system. However, excessive perchlorate (5800 mg/L) would exceed the treatment capacity of AnGS, which resulted in the deterioration of removal performance. This work provided an effective information for AnGS application to treat high-strength perchlorate wastewater.Biochar, has recently, been widely used as a potential soil additive to improve the quality of cultivated land. However, the effect of biochar on irrigation water use efficiency (IWUE) remains unclear in the Qinghai-Tibet Plateau (QTP). Therefore, the purpose of this study was to explore the effects of biochar on the soil properties, water infiltration, and irrigation water efficiency of QTP cultivated land. A column experiment with four biochar application levels (0, 3, 6, and 9 kg·m-2 denoted CK, BC1, BC2, and BC3, respectively) was conducted to explore the biochar effect on the soil water infiltration process. The soil bulk density (γ), saturated water content (θs), soil water retention curve (SWRC), specific water capacity C(h), and saturated hydraulic conductivity (Ks) were measured after the trial. The effects of biochar application level, biochar application depth, irrigation water depth, and initial soil moisture on water loss and IWUE were then simulated by HYDRUS-2D. The results showed that biochar slowed the process of soil water infiltration by changing the soil physical properties and hydraulic properties, reducing the water loss by 5%-15.02%, effectively alleviating the waste of irrigation water, and therefore increasing IWUE by 2%-9.43%. Water loss and IWUE were significantly associated with the biochar application depth and level. Additionally, a biochar level of 6 kg·m-2 showed the best effect for ameliorating the QTP’s cultivated soil. These results provide a novel approach for reducing water loss and enhancing the irrigation water use efficiency of QTP cultivated soil.In this article, we propose that ecosystem services (ES) should be studied integrating social participation and the narrative of social actors. We analyzed the ES literature (1996-2020) in Latin America (LA), basing our review on the concept that the study of this topic should be transdisciplinary and post-normal (i.e., extended peer communities). We prepared the review using the Scopus® and Web of Science™ (WoS) databases. We found 1069 articles related to social participation in ES studies in 20 LA countries, identifying 310 articles for further analysis using screening and eligibility protocols. We also used a random sample (n = 50) of the 310 articles for a detailed analysis of social participation and extended peer communities. Results showed that articles increased from seven in 2010 to 39 per year from 2015 to 2019. English is the primary language used (91% of the articles), with only one journal accepting publications in Spanish. The most common collaboration combination has been one LA author and one or more non-LA authors (41% of the articles). The semantic network analysis showed 35 thematic clusters, with the most common corresponding to ES protection and provision issues. Direct social participation was included in 62% of the articles, mainly through interviews; however, consultancy processes have dominated the participatory perspective of the authors without transformative involvement. We discuss article language and low inter-countries collaboration, both influencing the lack of social participation required for the transdisciplinary analysis of ES.

Over the years, various epidemiological studies found that acute myocardial infarction (AMI) often shows seasonal rhythm patterning, which is usually influenced by the variations of environmental factors, such as air pollution, ambient temperature, solar activity, relative humidity. However, there are few studies on the impact of sunlight-induced AMI especially in developing countries, and they had inconsistent results. This study aimed to examine within-summer variations in the temporal association between sun exposure and AMI.

We obtained hospitalization data for AMI of Beijing during 2013-2019. We used a distributed lag non-linear model (DLNM) combined with a quasi-Poisson regression model to estimate the non-linear lag effects of sunshine duration on AMI incidences. We evaluated the overall effect of AMI admissions with exposure to sunshine duration in the lag 0-21 days.

A total of 45,301 AMI cases were enrolled in our study during summer (June-September). The minimum of the morbidity was during daye, and provide appropriate public health recommendations accordingly to gain the greatest benefits from sunlight.Large airport operation release harmful air pollutants and have adverse impact on local air quality. As one of the world’s top 30 busiest airports, Xinzheng International Airport (CGO) located in Zhengzhou City, China, its emission impacts needs particular attention. To identify the possible impacts and quantify the contribution of CGO airport to air pollution, a comprehensive approach including emission inventory, continuous monitoring, coupled with statistical modelling was adopted in this study. We estimated a more detailed emissions inventory for CGO, including hourly and annual emissions from engines and auxiliary power units of aircrafts during landing and take-off flight, and airside ground support equipment (GSE) in 2019. The results indicate that almost all the CGO specific parameters including operating hours, fuel consumption and unit LTO emissions at different modes were lower than ICAO reference values. The annual total emissions of NOx, CO, HC, SO2 and PM from CGO from aircrafts and GSE were 1207.7, 921.3, 123.7, 268.3 and 36.2 tons, respectively. In addition to SO2, the main engines of the aircraft accounted for 80.3%, 62.6%, 45.5% and 74.3% of the total emissions, respectively. Meanwhile, a continuous monitoring campaign was conducted for one year in the vicinity of CGO airport. The monitoring data were analyzed using generalized additive model (GAM) to quantify the impact of NOx emissions from airport activities on air quality at CGO. The results showed that even the influence of environmental and meteorological variables was greater, nearly 13% of the ambient NOx concentrations were explained by emissions from airport activities, indicating the importance of airport-related emissions as the major source affecting local air quality.Per- and polyfluoroalkyl substances (PFAS) comprise a wide group of persistent chemicals, whose ubiquitous occurrence in the environment, particularly due to their extensive use for fire suppression in aqueous film forming foams (AFFFs), has raised global attention. We evaluated the impacts of PFAS at three firefighting training sites and one industrial site in Finland, to highlight key elements to be considered in the retrospective risk assessment of these chemicals. The site assessments covered the occurrence and distribution of 23 PFAS in multiple environmental matrices, i.e., soil, sediment, surface water, groundwater, and biota, and the subsequent risks to human health and the environment owing to the migration of and exposure to the selected compounds. Our study showed that the extensive use of nowadays restricted or substituted PFAS, particularly PFOS, are still often the predominant compounds detected at AFFF-impacted sites and will continue to cause long-term risks to the environment. The most significant environmental or health risks at these sites are likely to concern aquatic ecosystems, fish consumption or groundwater usage due to the off-site migration of PFAS. Here, even a single fire extinguishing event can be a considerable contributor. We also illustrate that conventional procedures based on simple mass-balance, and exposure models, with a focus on PFOS and other site-specifically relevant PFAS may provide sufficient means to assess the risks. Moreover, we address that despite the exceedance of the very stringent regulatory threshold values issued recently for PFAS, the actual site-specific risks to human health and the environment may remain reasonably low.Constructed wetland (CW) is a cost-effective and environmentally friendly ecological technology for contaminated water remediation, especially in dispersed communities and rural areas. Plants grow, biofilms form, and pollutants attach to the substrate, which is the main supporting structure of a subsurface flow CW (SSFCW) system. After long-term operation, the accumulation of clogs from physical, chemical, and biological processes in SSFCW substrates can easily cause clogging, thus reducing treatment efficiency reduction and service life and causing no discharge of sewage by intermittent until last indicates in the CW surface. Subsequently, stench and mosquito breeding occur, thus influencing environmental sanitation. Substrate clogging is the most serious, challenging, and inevitable problem in the long-term operation of SSFCWs. The present study reviews the effects of substrates on clogging categorized into physical, chemical, and biological clogging and analyzes the substrates that can alleviate/aggravate clogging in CWs. The recommended substrates that can relieve clogging include plastic, rubber, soil mixture, walnut shell, biochar, organic waste, alum sludge, and lightweight aggregate, while shell, steel slag, blast furnace slag, zeolite, and soil may easily generate phosphorus-clogging substances. CW substrate clogging is a mixture of three clogs with synergistic effects, and the corresponding clogging mitigation substrates mentioned above can be used to alleviate the most severe among the three types of clogs to reduce the synergy, and thus to promote stable operation and technology level of CWs. This review aims to promote the scientific selection of substrates for the stable operation and technical level of CW through targeted recommendations for substrates that relieve clogging. Future studies should focus the effects of influent water quality and substrate type on clogging, and waste as substrate to alleviate clogging, while mitigating the negative environmental impact of waste treatment.Nitrogen removal from landfill leachate (LL) using microalgae is a promising method and can realize CO2 mitigation. But the performances are usually inhibited by high chromaticity, high free ammonia (FAN) and some complex macro molecular organic matter (MOM) in the LL. To achieve efficient nitrogen removal from LL, this study firstly pretreated the mature LL with ozone, decolorizer and activated sludge (AS) respectively, and then inoculated with mixed microalgae. The results showed that the synergistic effect of ozonation and microalgae was the best among the three, with 99.7% ammonia removal, 0.77 g/L (dry weight) microalgae biomass, and a maximum growth rate of 160 mg/L/d. Ozonation pretreatment significantly reduced the chromaticity and macromolecular organic matter of LL, with the chromaticity reduced from 2225 to 225 times and the 3D fluorescence intensity representing MOM reduced from 4089 a.u. to 986.1 a.u.. And it was found that the mixed microalgae grown after pretreatment by three different methods all were mostly Chlorella and very few Microcystis, and the density of microalgal populations (number of cells per unit volume) after ozonation was up to 10,650 cells/μL. This work provides a feasible and an economical way to remove ammonia nitrogen (NH+ 4-N) from landfill leachate.Contribution of liquid water content (LWC) to the levels of the carcinogenic particulate nitro(so) compounds and the chemistry affecting LWC were investigated based on the observation of seven nitrosamines and two nitramines in rural (Seosan) and urban (Seoul) area in South Korea during October 2019 and a model simulation. The concentrations of both the total nitrosamines and nitramines were higher in Seosan (12.48 ± 16.12 ng/m3 and 0.65 ± 0.71 ng/m3, respectively) than Seoul (7.41 ± 13.59 ng/m3 and 0.24 ± 0.15 ng/m3, respectively). The estimated LWC using a thermodynamic model in Seosan (12.92 ± 9.77 μg/m3) was higher than that in Seoul (6.20 ± 5.35 μg/m3) mainly due to higher relative humidity (75 ± 9% (Seosan); 62 ± 10% (Seoul)) and higher concentrations of free ammonia (0.13 ± 0.09 μmol/m3 (Seosan); 0.08 ± 0.01 μmol/m3 (Seoul)) and total nitric acid (0.09 ± 0.07 μmol/m3 (Seosan); 0.04 ± 0.02 μmol/m3 (Seoul)) in Seosan while neither fog nor rain occurred during the sampling period. The relatively high concentrations of the particulate nitrosamines (>30 ng/m3) only observed probably due to the higher LWC (>10 μg/m3) in Seosan. It implies that aqueous phase reactions involving NO2 and/or uptake from the gas phase enhanced by LWC could be promoted in Seosan. Strong correlation between the concentrations of nitrosodi-methylamine (NDMA), an example of nitrosamines, simulated by a kinetic box model including the aqueous phase reactions and the measured concentration of NDMA in Seosan (R = 0.77; 0.37 (Seoul)) indicates that the aqueous phase reactions dominantly enhanced the NDMA concentrations in Seosan. On the other hand, it is estimated that the formation of nitrosamines by aqueous phase reaction was not significant due to the relatively lower LWC in Seoul compared to that in Seosan. Furthermore, it is presumed that nitramines are mostly emitted from the primary emission sources. This study implies that the concentration of the particulate nitrosamines can be promoted by aqueous phase reaction enhanced by LWC.

Some evidence suggests that teeth treated with endodontic surgery and considered to have healed over the short term are seen to relapse when evaluated again after 3 or more years. However, long-term evidence is limited. This study compares healing after endodontic microsurgery over long-term (5-9 years) vs middle-term (1-4 years) follow-up and assesses the influence of different healing predictors over time.

A retrospective study was made, comparing the endodontic microsurgery healing rates after 1-4 vs 5-9years of follow-up. Healing was assessed based on clinical and radiographic parameters. Simple binary logistic regression models were used to analyze the influence of patient age and gender, the type of tooth, previous radiographic lesion size, apical extent of previous root canal filling, the presence of a post, type of restoration, and interproximal bone level upon the endodontic microsurgery healing rate. A sensitivity analysis was used excluding cases of vertical root fracture. Two calibrated observ of the tooth, being significantly poorer when probing depth was >3mm mesial or distal to the treated tooth.

3 mm mesial or distal to the treated tooth.BTB and CNC homology 1 (Bach1) is a protein that forms nuclear heterodimers with the small musculoaponeurotic fibrosarcoma (sMaf). These bind to genomic DNA, promoting the inhibition of the synthesis of a range of antioxidant enzymes. This heterodimer antagonises the actions of nuclear factor erythroid 2-related factor-2 (Nrf2), a master regulator of cytoprotective responses in the cells. Studies have shown that Nrf2 expression is downregulated and Bach1 expression upregulated in many chronic diseases; hence Nrf2 activators and Bach1 inhibitors need to be investigated for their potential to mitigate inflammation and improve antioxidant responses in the chronic burden of lifestyle diseases, including chronic kidney disease. Thus, this review will discuss the status of Bach1 in such diseases and the use of possible inhibitors as a promising therapeutic approach.Chemical leukoderma is an acquired depigmentation of the skin caused by repeated exposure to specific agents damaging to epidermal melanocytes. Case reports of chemical leukoderma have been associated with some consumer products. To date, there are no well-accepted approaches for evaluating and minimizing this risk. To this end, a framework is presented that evaluates the physical and chemical characteristics of compounds associated with chemical leukoderma and employs structure-activity relationship (SAR) read-across and predictive metabolism tools to determine whether a compound is at increased risk of evoking chemical leukoderma. In addition to in silico approaches, the testing strategy includes in chemico quinone formation and in vitro melanocyte cytotoxicity assays to dimension the risk as part of an overall weight of evidence approach to risk assessment. Cosmetic ingredients raspberry ketone, undecylenoyl phenylalanine, tocopheryl succinate, p-coumaric acid, resveratrol, resveratrol dimethyl ether, sucrose dilaurate, tranexamic acid, niacinamide and caffeic acid are evaluated in this framework and compared to positive controls rhododendrol and hydroquinone. Overall, this framework is considered an important step toward mitigating the risk of chemical leukoderma for compounds used in consumer products.

Odor identification (OI) impairment increases the risk of Alzheimer’s disease and brain abnormalities in patients with late-life depression (LLD). However, it remains unclear whether abnormal functional connectivity (FC) of olfactory regions is involved in the relationship between OI impairment and dementia risk in LLD patients. The current study aims to explore the olfactory FC patterns of LLD patients and how olfactory FCs mediate the relationship between OI and cognition.

A total of 150 participants underwent resting-state functional magnetic resonance imaging and psychometric and olfactory assessments. The primary and secondary olfactory regions were selected as regions of interest to investigate olfactory FC patterns and their association with OI and cognitive performance in LLD patients.

Compared with LLD patients without OI impairment and normal controls, LLD patients with OI impairment exhibited increased FC between the left orbital frontal cortex (OFC) and left calcarine gyrus, between the leftwith intact OI, and these abnormal FCs may serve as potential targets for neuromodulation in LLD patients to prevent them from developing dementia.

Stochastic resonance (SR) is achieved when a faint signal is improved with the addition of the appropriate amount of white noise. Perceptual thresholds are expected to follow a characteristic performance improvement curve as a function of the white noise level added (i.e., thresholds are reduced with an optimal amount of added white noise, beyond which excessive white noise is no longer beneficial). Since SR exhibition in perceptual thresholds is defined by a shape rather than a statistical difference, the presence of SR is typically identified qualitatively. The current state-of-the-art is for blinded human judges to categorize the presence of SR by visually examining data. While categorizations are made with subject data intermixed within a balanced, simulated dataset, which accounts for false positives, this method is still subjective and prone to human error.

We use a logistic regression (LR) trained on engineered features in order to quantitatively classify exhibition of SR. The LR was trained on datasets simulated from a model for SR performance enhancement.

We implemented the algorithmic classification process in 6 perceptual threshold test cases, informed by the literature and parameters were defined by experimental subject data. Comparison to Existing Method(s) We report algorithmic classifications of SR exhibition, considering the 6 test cases, that outperform existing subjective methods in accuracy (p<0.05).

We demonstrate that algorithmic classification can effectively identify SR in perceptual thresholds, providing a rigorous, objective, and quantitative approach to identifying the presence of SR.

We demonstrate that algorithmic classification can effectively identify SR in perceptual thresholds, providing a rigorous, objective, and quantitative approach to identifying the presence of SR.

Photothermal stimulation is a heat-mediated neuromodulation technique. When photothermal effects are induced on neuronal membrane, it can either excite or inhibit neural spiking activities. It has been demonstrated that gold nanorod mediated photothermal stimulation could decrease the electrical activity of cultured neural network. We investigated the effect of photothermal inhibition on neural activity using calcium imaging technique.

Hippocampal neurons were cultured on a gold-nanorod-coated-microelectrode array and near-infrared laser was illuminated to induce neural inhibition. The neuronal responses at a single-cell resolution were measured by an extracellular recording and calcium imaging simultaneously.

The photothermal effect on neural spikes were confirmed by electrical recording and calcium imaging. The decrease in neural spikes in electrical recordings during NIR illumination was correlated with the neighboring neural activity quantified by calcium spikes.

Optical recording at the single cell resolution was attempted during photothermal stimulation to confirm the neural suppression effect.

Heat mediated suppression of neural activity was optically validated in single cell level. The present study will be helpful to understand the emerging photothermal neuromodulation technology.

Heat mediated suppression of neural activity was optically validated in single cell level. The present study will be helpful to understand the emerging photothermal neuromodulation technology.

The rodent vibrissal (whisker) systcnsorimotor integration and active tactile sensing. Experiments on the vibrissal system often require highly repeatable stimulation of multiple whiskers and the ability to vary stimulation parameters across a wide range. The stimulator must also be easy to position and adjust. Developing a multi-whisker stimulation system that meets these criteria remains challenging.

We describe a novel multi-whisker stimulator to assess neural selectivity for the direction of global motion. The device can generate repeatable, linear sweeps of tactile stimulation across the whisker array in any direction and with a range of speeds. A fiber optic beam break detects the interval of whisker contact as the stimulator passes through the array.

We demonstrate the device’s function and utility by recording from a small number of multi-whisker-responsive neurons in the trigeminal brainstem. Neurons had higher firing rates in response to faster stimulation speeds; some also exhibited strong direction-of-motion tuning.

The stimulator complements more standard piezo-electric stimulators, which offer precise control but typically stimulate only single whiskers, require whisker trimming, and travel through small angles. It also complements non-contact methods of stimulation such as air-puffs and electromagnetic-induced stimulation. Tradeoffs include stimulation speed and frequency, and the inability to stimulate whiskers individually.

The stimulator could be used – in either anesthetized or awake, head-fixed preparations – as an approach to studying global motion selectivity of multi-whisker sensitive neurons at multiple levels of the vibrissal-trigeminal system.

The stimulator could be used – in either anesthetized or awake, head-fixed preparations – as an approach to studying global motion selectivity of multi-whisker sensitive neurons at multiple levels of the vibrissal-trigeminal system.

Transcription factor-based forward programming enables the efficient generation of forebrain excitatory and inhibitory neurons from human pluripotent stem cells (hPSCs). This provides an opportunity to study stimulation-response patterns in highly defined neuronal networks in a controlled and customizable in vitro environment.

Cell populations composed of defined ratios of excitatory and inhibitory neurons were generated by forward programming genome-edited human hPSCs carrying the inducible transcription factors NGN2 and ASCL1/DLX2, respectively. These populations were cultured on multi-electrode arrays (MEAs), and population responses elicited by distinct spatial and temporal stimulation patterns were analyzed. In parallel, in silico network models fed with neuronal parameters obtained from the in vitro cultures were developed to explore potential mechanisms underlying experimental observations.

Neuronal cultures developed network-level electrophysiological activities with pronounced synchronized netwonses to electric stimuli. It also illustrates how the combined use of biological and in silico neuronal networks can support the development of mechanistic hypotheses underlying network responses to specific stimuli.

The present study demonstrates the prospect of the iPSC technology for conducting personalized in vitro studies of human neuronal networks and their responses to electric stimuli. It also illustrates how the combined use of biological and in silico neuronal networks can support the development of mechanistic hypotheses underlying network responses to specific stimuli.Marketing pressure on teenagers when it comes to promoting unhealthy foods and food brands is a significant public health concern. Teenagers are aggressively targeted by food marketing messages, yet a research gap exists when it comes to the engagement by teens with this marketing in real world settings, and specific techniques (or power) used to capture their attention. This exploratory study engages in participatory research to explore the persuasive power and platforms of exposure of teen-targeted food marketing. Using an innovative smartphone app called „GrabFM!” („Grab Food Marketing!”), teens ages 13-17 (n = 62) identified and tagged examples (n = 339) of targeted food marketing (from mainstream and digital media, and the built environment) over a 7-day period, providing information on the food brand, product, platform, and indicators (i.e., persuasive techniques). Results revealed the top brand (FritoLay, 8.3%), food product category (candy/chocolate, 23.3%), platform of exposure (Instagram, 76.4%), and indicator (visual style, 52.5%) identified by teens. Insights were also gained into the intersection of gender and platform, gender, age and indicators (older teens 15+ more likely to report multiple indicators per ad), and co-occurrence of indicators (majority of ads tagged with one indicator only). The results of this study provide guidance on the power, platforms and brands that teens felt uniquely spoke to them. When it comes to monitoring efforts, it is useful to know that Instagram commands teenagers’ attention and that marketing power resides in particular indicators (visual style, special offer, theme), which teenagers appear to readily and consistently identify.The P3 component (P300, P3b) is considered to be an effective index of attention and categorization processes when elicited in a visual oddball task, specifically reflecting the selection of a rare target item among frequent non-targets. Researchers have proposed that target categorization is guided by representations of target features held in working memory (WM), thus guiding attention and categorization processes to distinguish targets from non-targets. Although WM is theorized to have visuospatial, verbal and executive function components, most studies do not investigate how these WM components contribute to the P3. This study uses an individual differences approach to determine whether correlations between WM capabilities and P3 amplitudes indicate a common underlying cognitive construct. Participants (n = 140) completed an 80/20 visual oddball task to elicit the P3 as well as independent visual working memory (VWM), spatial working memory (SPWM), and executive function (task switching (TS) and digit symbol substitution (DSS)) tests. Results indicated that measures of executive function, DSS and TS, but not VWM or SPWM ability, correlated with and predicted faster task response times and greater P3 amplitudes. RT and WM measures were not correlated with P3 fractional area latencies. These results support context updating theory. Executive function WM availability, whether as a property of the participant’s processing system or based on task demands, plays a functional role in the P3 and an important role in efficient visual categorization and goal-directed learning.

To describe characteristics, hospitalization, and death for reported cases of SARS-CoV-2 infection in the Oklahoma City tri-county area.

We extracted notified cases of SARS-CoV-2 infection for our study area and used descriptive statistics and modeling to examine case characteristics and calculate the odds of hospitalization and death in relation to a range of explanatory variables.

Between March 12th, 2020 and February 28th, 2021, 124,925 cases of SARS-CoV-2 infection were reported from the study region. Being male, White or Black/African American, aged 50 years or older, presenting with apnea, cough, and shortness of breath, and having diabetes was associated with increased odds of hospitalization. The odds of dying were significantly associated with being Black/African American, presenting with cough and fever, having kidney disease and diabetes and being aged 70 years or older.

The first cohort analysis of SARS-CoV-2 positive individuals in the Oklahoma City tri-county area confirms comorbidities and age as important predictors of COVID-19 hospitalization or death. As a novel aspect, we show that early symptoms of breathing difficulties in particular are associated with hospitalization and death. Initial case assessment and SARS-CoV-2 guidelines should continue to focus on age, comorbidities, and early symptoms.

The first cohort analysis of SARS-CoV-2 positive individuals in the Oklahoma City tri-county area confirms comorbidities and age as important predictors of COVID-19 hospitalization or death. As a novel aspect, we show that early symptoms of breathing difficulties in particular are associated with hospitalization and death. Initial case assessment and SARS-CoV-2 guidelines should continue to focus on age, comorbidities, and early symptoms.

Medical students’ white coats were found to harbor harmful organisms. This could be due to non-compliance to white coat hygiene measures. Therefore, we aim to develop and validate a questionnaire to assess the of knowledge, attitude, and practice (LAUNDERKAP) of white coat use among medical students in Malaysia.

This study was conducted in 4 local medical schools. LAUNDERKAP was developed via literature review and had 3 domains attitude, knowledge, practice. An expert panel assessed the content validity and clarity of wording. LAUNDERKAP was then piloted among 32 medical students. To test construct validity and internal consistency, 362 medical students were approached. Construct validity was assessed using exploratory factor analysis. Internal consistency was evaluated using Cronbach alpha for attitude and practice, while Kuder-Richardson 20 (KR-20) was used for knowledge.

A total of 319 of 362 students responded. Exploratory factor analysis extracted 1 factor each for attitude and knowledge respectively, and 3 factors for practice. Cronbach alpha for attitude was 0.843 while KR-20 for knowledge was 0.457. Cronbach alpha for practice ranged from 0.375 to 0.689. The final LAUNDERKAP contained 32-items (13 attitude, 9 knowledge, 10 practice).

LAUNDERKAP had adequate psychometric properties and can be used to assess KAP of medical students towards white coat use.

LAUNDERKAP had adequate psychometric properties and can be used to assess KAP of medical students towards white coat use.

To identify key socio-demographic, lifestyle, and health predictors of self-reported coronavirus disease 2019 (Covid-19) history, examine cardiometabolic health characteristics as predictors of self-reported Covid-19 history and compare groups with and without a history of Covid-19 on trajectories in cardiometabolic health and blood pressure measurements over time, among United States (U.S.) older adults.

Nationally representative longitudinal data on U.S. older adults from the 2006-2020 Health and Retirement Study were analyzed using logistic and mixed-effects logistic regression models.

Based on logistic regression, number of household members (OR=1.26, 95% CI 1.05, 1.52), depressive symptoms score (OR = 1.21, 95% CI 1.04, 1.42) and number of cardiometabolic risk factors or chronic conditions („1-2” vs „0”) (OR = 0.27, 95% CI 0.11, 0.67) were significant predictors of self-reported Covid-19 history. Based on mixed-effects logistic regression, several statistically significant predictors of Covid-19 history were identified, including female sex (OR = 3.06, 95% CI 1.57, 5.96), other race (OR = 5.85, 95% CI 2.37, 14.43), Hispanic ethnicity (OR = 2.66, 95% CI 1.15, 6.17), number of household members (OR = 1.25, 95% CI 1.10, 1.42), moderate-to-vigorous physical activity (1-4 times per month vs never) (OR = 0.38, 95% CI 0.18, 0.78) and number of cardiometabolic risk factors or chronic conditions („1-2” vs „0”) (OR = 0.34, 95% CI 0.19, 0.60).

Number of household members, depressive symptoms and number of cardiometabolic risk factors or chronic conditions may be key predictors for self-reported Covid-19 history among U.S. older adults. In-depth analyses are needed to confirm preliminary findings.

Number of household members, depressive symptoms and number of cardiometabolic risk factors or chronic conditions may be key predictors for self-reported Covid-19 history among U.S. older adults. In-depth analyses are needed to confirm preliminary findings.

To assess the safety and efficacy of modified testicular traction technique in treatment of intraabdominal and peeping testes with short vessels.

In the pediatric surgery unit, Sheikh Khalifa Medical City, Abu Dhabi, UAE, and in the pediatric urology unit, Ibn Sina Hospital, Kuwait, Forty testes in 32 patients were operated using the modification of staged traction laparoscopic-orchiopexy. The modified technique involved intracorporeal placement of suture, gabernacular sparing, no intraabdominal dissection and shorter traction duration as short as 7 days without the need of inguinal incision.

All patients tolerated both stages of surgery well without any intra-operative nor post-operative complications. The 1 year follow up period proved successful outcome in all the cases.

The modified staged traction laparoscopic orchiopexy is safe, successful and feasible in intra-abdominal and peeping testes.

The modified staged traction laparoscopic orchiopexy is safe, successful and feasible in intra-abdominal and peeping testes.

To evaluate the impact of personality traits and institutional factors on burnout among a population of practicing urologists.

From 2017-2019 a voluntary survey was distributed to practicing urologists across the United States. The survey evaluated demographics, education, social factors, practice types and work satisfaction. Personality traits were evaluated using the Sheffield psychometric assessment. Burnout was assessed using the Maslach inventory and defined as a score of ≥27 on the emotional exhaustion domain or ≥10 on the depersonalization domain.

One hundred seventy-three urologists responded, of whom 86.7% were male, 88.1% Caucasian, and 53.5% self-identified as general urologists. 49.1% (85/173) met criteria for burnout. On univariate analysis, burnout was associated with taking call, a ≥51 hour/week work schedule, and using multiple EMRs. On the psychometric assessment, tendencies towards the personality traits resiliency, optimism, extroversion, and a team player approach were associated witork hours were universally associated with increased burnout. Awareness of these risks and relationships can help develop strategies to identify and curtail burnout.To examine the association between type of analgesic block and incidence of complications following primary hypospadias correction. Data sources included MEDLINE, Embase, Web of Science and the Cochrane Library, inception-01/2021. Randomized clinical trials, cohort and case control studies reporting original data for patients less then 18 years of age undergoing primary hypospadias correction with either a penile or caudal block for which outcomes (urethrocutaneous fistula or glans dehiscence) were reported. Two researchers independently extracted data and assessed quality for inclusion. The primary outcome was the incidence of complication within six-months postoperatively based on block performed. Ten studies (3201 patients; range 54-983) were included. Six studies (cumulative weight 28.6%) favored penile block while 4 studies (cumulative weight 71.4%) favored caudal block. Compared to the reference group of penile blocks, caudal blocks had no significant association with development of complications following primary hypospadias correction (relative risk 1.11, 95% CI (0.88, 1.41); P = .38). When adjusting for meatal location (distal vs proximal) there was no significant association with development of fistulae or glanular dehiscence following primary hypospadias correction with caudal blocks in comparison to the reference group, penile blocks for distal, (relative risk 1.46, 95%CI (0.98, 2.17); P = .065) and proximal (relative risk 0.95, 95% CI (0.58, 1.54); P = .823). The type of analgesic block is not associated with the risk of developing complications following primary hypospadias correction in children. Caudal block should be considered for these urological interventions.Peyronie’s Disease (PD) is estimated to occur in up to 13% of males and has been associated with Dupuytren’s Disease (DD). We identified 3 men with PD/DD and hypothesized that there may be a genetic association between the 2 diseases. Blood samples were collected from the participants and sent for whole genome sequencing. A rare non-synonymous mutation in the ALMS1 gene was identified in 3 men. Interestingly, ALMS1 is associated with TGF-b, and aberrant fibrosis. This pilot study generates the hypothesis that mutations in ALMS1 may predispose patients to development of PD/DD.Canine parvovirus 2 (CPV-2), a highly contagious virus, affects dogs worldwide. Infected animals present severe and acute gastroenteritis which may culminate in death. CPV-2 VP2 protein is responsible for important biological functions related to virus-host interactions. Herein we obtained VP2 full-length gene sequences from Brazilian dogs with bloody diarrhea (n=15) and vaccine strains (n=7) produced by seven different laboratories and marketed in Brazil. All wild sequences and one vaccine strain were classified as CPV-2b and six vaccines were the classic CVP-2. Mutations in VP2 protein from vaccine and wild strains obtained in Brazil and worldwide were analyzed (n=906). Amino acid sequences from vaccine strains remarkably diverge from each other, even that classic CPV-2. Phylogenetic analysis based on VP2 gene and conducted with sequences displaying mutations in epitope regions previously described shows that vaccine strains are distantly related from the wide range of wild CPV-2. The impact of amino acid mutations over VP2 protein structure shows that vaccine and wild strains obtained in this study diverge in loop 3, an epitope region that plays a role in the CPV-2 host range. This is the first analysis of CPV-2 VP2 from commercial vaccine strains in Brazil and wild ones from Minas Gerais State, Brazil, and the first detailed attempt to vaccinal VP2 molecular and structural analyses.Global analysis of the susceptibility of influenza viruses to neuraminidase (NA) inhibitors (NAIs) and the polymerase acidic (PA) inhibitor (PAI) baloxavir was conducted by five World Health Organization Collaborating Centres for Reference and Research on Influenza during two periods (May 2018-May 2019 and May 2019-May 2020). Combined phenotypic and NA sequence-based analysis revealed that the global frequency of viruses displaying reduced or highly reduced inhibition (RI or HRI) or potential to show RI/HRI by NAIs remained low, 0.5% (165/35045) and 0.6% (159/26010) for the 2018-2019 and 2019-2020 periods, respectively. The most common amino acid substitution was NA-H275Y (N1 numbering) conferring HRI by oseltamivir and peramivir in A(H1N1)pdm09 viruses. Combined phenotypic and PA sequence-based analysis showed that the global frequency of viruses showing reduced susceptibility to baloxavir or carrying substitutions associated with reduced susceptibility was low, 0.5% (72/15906) and 0.1% (18/15692) for the 2018-2019 and 2019-2020 periods, respectively. Most (n = 61) of these viruses had I38→T/F/M/S/L/V PA amino acid substitutions. In Japan, where baloxavir use was highest, the rate was 4.5% (41/919) in the 2018-2019 period and most of the viruses (n = 32) had PA-I38T. Zoonotic viruses isolated from humans (n = 32) in different countries did not contain substitutions in NA associated with NAI RI/HRI phenotypes. One A(H5N6) virus had a dual substitution PA-I38V + PA-E199G, which may reduce susceptibility to baloxavir. Therefore, NAIs and baloxavir remain appropriate choices for the treatment of influenza virus infections, but close monitoring of antiviral susceptibility is warranted.

Blood pressure control has been shown to reduce risk of vascular events and mortality after an ischemic stroke or transient ischemic attack (TIA). Yet, questions remain about effectiveness, timing, and targeted blood pressure reduction.

We analyzed data from a retrospective cohort of 18,837 veterans cared for 12 months prior and up to 12 months after an emergency department visit or inpatient admission for stroke or TIA. Latent class growth analysis was used to classify patients into systolic blood pressure trajectories. With Cox proportional hazard models, we examined relationships between blood pressure trajectories, intensification of antihypertensive medication, and stroke (fatal or non-fatal) and all-cause mortality in 12 months following the index event.

The cohort was classified into 4 systolic blood pressure trajectories 19% with a low systolic blood pressure trajectory (mean systolic blood pressure=116 mm Hg); 65% with a medium systolic blood pressure trajectory (mean systolic blood pressure=13rtension management after stroke or TIA, particularly for individuals with high or very high blood pressure trajectories.This present study reports a novel laboratory protocol, the glucose oxidase peroxidase (GOPOD)-coupled continuous assay (GcCA) which is based on the general principle that all cellulases generate β-d-glucose as one of their hydrolytic products. Resultantly, the β-d-glucose generated serves as a substrate to an auxiliary enzyme, GOPOD, coupled to the cellulase hydrolytic process, in a simultaneous secondary reaction which continuously quantifies the concentration of glucose generated as a measure of incremental cellulase activity in the laboratory. The protocol is initial hydrolytic rate-driven. Thus, making it very suitable for kinetic studies of pure cellulase samples in the laboratory. GcCA protocol is simple, rapid, specific, highly sensitive and accurate, non-laborious and saves cost in terms of time and materials. It is robust and dynamic and could be modified or adapted to suit either standard, 1000 μL cuvette or 96-well microtiter plate formats. Its optimized total assay reaction mixture of 500 μL constitutes a substrate, enzyme, buffer, distilled water and coupled GOPOD components. The GcCA protocol was validated for linearity, applicability, sensitivity, precision and compared with a standard and widely used assay method for optimized enzyme activity. It was further compared with the most widely used routine assay procedure for determination of reducing sugars, the DNS method. The GcCA protocol is a high throughput technique which enables the assay of over 120 samples in one day.Properties and applications of gold nanoparticles (AuNPs) depend on their characteristics which are intrinsically connected to the reducing and capping agents used in their synthesis. Although polysaccharides are commonly used for Au salt reduction, the control over the result is often limited. Here, the selectively dicarboxylated cellulose (DCC) and hyaluronate (DCH) with adjustable composition and molecular weight are used for the first time as reducing and capping agents for AuNPs preparation in an environmental friendly one-step synthesis. Mechanism of reduction and structure-function relationships between the composition of oxidized polysaccharides and properties of formed AuNPs are elucidated and the variances in the macromolecular architecture of dicarboxypolysaccharides are applied to guide the growth of AuNPs. While the homogenous structure and high density of carboxyl groups of fully-oxidized DCC induced isotropic growth of small and uniform AuNPs with good catalytic performance (d = ~20 nm, TOF = 7.