Wild yak (Bos mutus) is a vulnerable bovine species on the Qinghai-Tibetan Plateau (QTP). So far, most studies on molecular genetic diversity of wild yak have focused on autosomal and mtDNA variations based on small number of samples. In this study, we analyzed 84 D-loop and 24 whole mitogenome sequences of wild yak to further comprehensively explore its maternal genetic diversity and lineage composition. Meanwhile, using six yak Y-specific polymorphic markers (i.e., SRY4, USP9Y, UTY19, AMELY3, OFD1Y10 and INRA189), we assessed the paternal genetic diversity and lineage composition based on eight wild yak. Our results showed that wild yak exhibited abundant maternal genetic diversity with haplotype diversities of 0.9621 ± 0.0078 and 0.9928 ± 0.0144 in the D-loop and whole mitogenome sequences, respectively. Maternal phylogenetic analysis of wild yak uncovered three defined lineages (mt-I, mt-II and mt-III). Similarly, profuse paternal genetic diversity was observed in wild yak with Y-haplotype diversity (Hd) at 0.8214 ± 0.1007. Two Y-haplogroups (Y1 and Y2) with four Y-haplotypes (yH1-yH4) were identified in paternal phylogenetic analysis, indicating wild yak to be of two paternal lineages. This study of genetic diversity and lineage composition of wild yak would provide useful information for the genetic resource conservation and utilization of this vulnerable wild species.

The aim of this study was to determine the relationship between preoperative nutritional status and wound healing in patients undergoing surgical reconstruction for ischemic tissue loss.

The preoperative nutritional status of patients who underwent surgical reconstruction for ischemic tissue loss for the years 2011-2018 was retrospectively estimated using the Controlling Nutritional Status (CONUT) score. Patients were allocated to two groups Group I, normal nutrition or mild malnutrition (CONUT score ≤4), and Group II, moderate-to-severe malnutrition (CONUT score ≥5). Wound healing was set as the primary endpoint and major amputation and death as the secondary endpoints. The wound healing, limb salvage, and overall survival rates were calculated after two years using the Kaplan-Meier method, and Cox proportional hazards regression analysis was performed to determine which variables were independently associated with these outcomes.

Forty-eight patients with missing values for the CONUT score were excluded. A total of 174 limbs in 147 patients were studied (Group I 115 limbs in 100 patients; Group II 59 limbs in 47 patients). The mean duration of the study was 519 ± 270 days. The Kaplan-Meier curves showed that wound healing in Group I was superior to that in Group II (Group I vs. II two-year wound healing, 97% vs. 79%; time to 50% wound healing, 83 vs. 150 days,

<

0.001), and multivariate analysis showed that the CONUT score was an independent predictor of wound healing (hazard ratio, 0.63; 95% confidence interval, 0.41-0.98;

=

0.038).

Patients with better preoperative nutritional status are more likely to experience wound healing earlier than those with excessive malnutrition.

Patients with better preoperative nutritional status are more likely to experience wound healing earlier than those with excessive malnutrition.Two experiments demonstrate that eyewitnesses often falsely recognise an actor performing an action that had actually been performed by someone else, even if the action involves negative emotions and the actor in question had only appeared in emotionally neutral contexts. Participants viewed videos, each involving an actor performing a neutral (e.g. making oatmeal) or negatively valenced (e.g. killing a roach) action, and were asked to remember the events (Experiment 1) or to rate them for valence and arousal (Experiment 2). In both experiments, participants remembered negative actions better than neutral actions. Participants were also especially likely, however, to falsely recognise a different person performing a negative action. Experiment 2 revealed that this effect was modulated by the prior emotional contexts in which an actor had appeared. Participants were still just as likely, however, to falsely recognise an actor who had only appeared in neutral contexts now performing a negative action as they were to falsely recognise this actor performing a different neutral action. These results suggest that even individuals seen only in benign contexts can be falsely remembered as having participated in emotionally charged events (e.g. crimes).Physical activity is related with academic achievement in children. This cross-sectional study aimed to assess whether cardiorespiratory fitness (CRF) and executive function act as mediators of the association between moderate to vigorous physical activity (MVPA) and academic achievement. This study included 186 schoolchildren (9-11 years) from Cuenca, Spain. Sociodemographic variables, anthropometric variables, academic achievement, executive function (inhibition, cognitive flexibility and working memory), CRF (20-m shuttle run) and physical activity (by accelerometry) were measured. Serial mediation models were estimated using the Hayes´ PROCESS macro. The significant paths in the model mediating this relationship between MVPA and academic achievement were as follows MVPA → CRF → academic achievement (IE = 0.068, 95% CI [0.018; 0.127]; IE = 0.079, 95% CI [0.029; 0.144]; and IE = 0.090, 95% CI [0.032; 0.165], controlling for inhibition, cognitive flexibility and working memory, respectively), MVPA → CRF → inhibition → academic achievement (0.018, 95% CI [0.001; 0.047]) and MVPA → cognitive flexibility → academic achievement (0.087, 95% CI [0.012; 0.169]). The relationship between MVPA and academic achievement may not be direct but mediated by CRF, cognitive flexibility, and inhibition via CRF. Physical activity interventions to improve AA should be focused on improvements in CRF and executive function.This study characterised the thigh muscle-tendon unit length variations (MTUv) in elite alpine skiing. MTUv were modelled for seven muscles from knee and hip angles in 17 national team skiers during 1602 cycles (546 Slalom (SL), 908 Giant-Slalom (GS), 136 Super-Giant (SG) and 12 Downhill (DH) cycles). The biarticular rectus femoris showed a dynamic pattern on both legs, especially in SL. On the other side, vastii displayed a stable length on the inside leg in all disciplines (37-46% of the cycle), contrasting with their dynamic behaviour on the outside leg being quasi-static for only 3% (SL) to 27% (DH) of the cycle. SL showed the largest amplitude of MTUv followed by GS, SG and DH. For vastus lateralis, MTUv was ~60%Lr.s-1 in SL but only ~12%Lr.s-1 in DH. In SL, a fine desynchronisation between both joints led to nearly constant MTUv (slow lengthening lasting ~45-51% of the cycle) for the biarticular hamstrings separated by faster variations during turn switch from outside to inside leg. In summary, biarticular MTUv were not characterised by single-joint behaviours, suggesting that extrapolating contraction regimen from knee joint only is not accurate in alpine skiing.

There are limited methods to predict the therapeutic effect of immune checkpoint inhibitors (ICIs). The purpose of this study was to explore the value of circulating microparticles (MPs) in predicting thetherapeutic effects of immunotherapy.

A prospective study was conducted at the cancer center of PLA general hospital, including all patients with advanced non-small cell lung cancer (NSCLC) who were treated with pembrolizumab or nivolumab from December 2018 to December 2019. The patients were divided into an immune-related objective response (iOR) group and an immune-related disease progression (iPD) group.The numbers of total MPs, platelet-derived microparticles (PMPs) and T-lymphocyte-derived microparticles (T-LyMPs) at baseline and after immunotherapy were detected using a flow cytometer. Univariate analysis and multivariate logistic regression analysis were used to determine the independent influencing factors.

We identified 32 patients in the iOR group and 18 patients in the iPD group. No significaon, this study found for the first time that elevated circulating T-LyMPs were associated with disease progression in advanced NSCLC.

Systemic immune-inflammation index (SII), on the basis of lymphocyte, neutrophil and platelet counts had been published to be a good prognostic factor in coronary artery disease. Nevertheless, the prognostic value of Systemic immune-inflammation index (SII) in a condition of no-reflow phenomenon (NRP) remains inconsistent, we evaluated the SII as a simple calculated tool for predicting the NRP among patients with STEMI who underwent primary percutaneus coronary intervention (PCI).

510 consecutive acute STEMI patients who underwent primary PCI within 12 h from symptom onset from October 2015 to January 2020 were enrolled in our study. The receiver-operating characteristic (ROC) curve was used to determine the cut-off value of SII to predict the no-reflow. Multivariate stepwise logistic regression, including covariates found to have a significant association with NRP in univariate analysis, was used to identify independent predictors of no-reflow.

A ROC curve analysis showed that the best cut-off value of SII for predicting no-reflow was 1028, with sensitivity and specificity of 79% and 70, respectively (AUC, 0.839; 95% CI 0.797-0.881). An ROC curve comparison analysis was performed to compare the SII with NLR and PLR. Multivariate analysis revealed that SII ≥1028 value (OR = 6.622, 95% confidence interval (CI) 3.802-11.627,

 < .001), not using aspirin prior to admission (OR = 0.431, 95%CI 0.236-0.786,

 = .006), and CRP (OR = 1.004, 95%CI 1.001-1.008,

 = .041) were independent predictors related to occurrence of NRP after primary PCI in patients with acute STEMI.

SII levels are independently associated with the NRP in patients undergoing primary PCI for acute STEMI. High SII may be a promising indicator for the prediction of NRP in these patients.

SII levels are independently associated with the NRP in patients undergoing primary PCI for acute STEMI. High SII may be a promising indicator for the prediction of NRP in these patients.The SARS-CoV-2 pandemic has become a global threat. It has become very difficult to control the spreading of the virus. The virus is a RNA virus and the virulence of the virus is mediated by three virulence causing proteins, viz., Nsp1, Nsp3c and ORF7. So far the drug designing endeavors against the virus have been being targeted towards the spike protein which is responsible for the entry of the virus inside human host as well as the RNA dependent RNA polymerase. However, no effective treatment against the virus has so far been developed. In the present situation, an attempt has been made to target the virulence protein factor Nsp1 which binds to the 40S ribosomal subunit of the human host. We tried to target the Nsp1 by in-silico virtual screening of ligand libraries. We built the three dimensional structure of Nsp1 and used the structure to screen the ChEMBL drug library. We used molecular docking simulations of the top6 screened ligands with Nsp1 and subjected the liagnd-Nsp1 complexes to molecular dynamics simulations to analyze the behaviors of the ligands in a virtual cell.