These morbid conditions were partially ameliorated by simultaneous administration of anti-IL-1α and β antibodies. The skin not only constitutes a physical barrier, but also functions as the largest immune organ. We suggest a novel role for IL-1 in the pathogenesis of EB and the use of anti-IL-1 antibodies as a potential therapy for EB complications.Nanoparticles (NPs) have unique properties compared to their bulk counterparts, and they have potentials for various applications in many fields of life science. Green-synthesized NPs have garnered considerable interest due to their inherent features such as rapidity, eco-friendliness and cost-effectiveness. Zinc oxide nanoparticles (ZnO NPs) were synthesized using an aqueous extract of Kalanchoe blossfeldiana as a reducing agent. The resulting nanoparticles were characterized via X-ray diffraction (XRD), dynamic light scattering (DLS), UV-Vis spectroscopy, photoluminescence (PL), transmission electron microscopy (TEM), scanning electron microscopy (SEM) and energy-dispersive spectroscopy (EDS). The antimicrobial potential of the synthesized ZnO NPs against bacterial and fungal strains was examined by the disk diffusion method, and they showed a promising antibacterial and antifungal potential. The catalytic activity of the synthesized ZnO NPs in reducing methylene blue (MB) and eosin was studied via UV-Vis spectroscopy. The decolorization percentages of the MB and Eosin Y dyes were 84% and 94%, respectively, which indicate an efficient degradation of the ZnO NPs. In addition, the cytotoxic activity of the ZnO NPs on the HeLa cell line was evaluated via in vitro assay. The MTT assay results demonstrate a potent cytotoxic effect of the ZnO NPs against the HeLa cancer cell line.Tilt and decentration of intraocular lenses (IOL) may occur secondary to a complicated cataract surgery or following an uneventful phacoemulsification. Although up to 2-3° tilt and a 0.2-0.3 mm decentration are common and clinically unnoticed for any design of IOL, larger extent of tilt and decentration has a negative impact on the optical performance and subsequently, the patients’ satisfaction. This negative impact does not affect various types of IOLs equally. In this paper we review the methods of measuring IOL tilt and decentration and focus on the effect of IOL tilt and decentration on visual function, in particular visual acuity, dysphotopsia, and wavefront aberrations. Our review found that the methods to measure the IOL displacement have significantly evolved and the available studies have employed different methods in their measurement, while comparability of these methods is questionable. There has been no universal reference point and axis to measure the IOL displacement between different studies. A remarkably high variety and brands of IOLs are used in various studies and occasionally, opposite results are noticed when two different brands of a same design were compared against another IOL design in two studies. We conclude that 0.5 mm. The effect of IOL displacement on visual function is more pronounced in aberration correcting IOLs compared to spherical and standard non-aberration correcting aspherical IOLs and in multifocal versus monofocal IOLs. Internal coma has been frequently associated with IOL tilt and decentration, and this increases with pupil size. There is no correlation between spherical aberration and IOL tilt or decentration. Although IOL tilt produces significant impact on visual outcome in toric IOLs, these lenses are more sensitive to rotation compared to tilt.Outbreaks of severe acute respiratory syndrome (SARS) and coronavirus disease 2019 (COVID-19) have affected populations worldwide. Our literature review summarises the studies reporting psychological issues among healthcare staff and infected patients in mainland China, Hong Kong, and Taiwan during these two outbreaks and the potential strategies for addressing these issues. Our review shows that patients and healthcare staff presented similar psychological symptoms, including anxiety, fear, distress, and depression, which may lead to stress-related complications such as insomnia. In patients, these psychological impairments can be contributed to by being quarantined, perceptions of threats to life, and uncertainty about health status. Quarantine is also a factor for distress among healthcare staff, together with their heavy workload, the fear that they and their families would become infected, witnessing their patients’ poor and deteriorating conditions, and the requirement to wear protective gear. Strategies that are needed to address these factors include providing counselling services, implementing mindfulness-based therapies and optimism interventions, and providing telecommunication facilities for patients to communicate with their families. Healthcare staff should also be provided with these services, together with appropriate and flexible work shift arrangements and morale boosting. These strategies would improve not only the mental well-being of patients and healthcare staff, but also the self-efficacy and competence of the staff to provide quality healthcare services.Soil salinity is the main obstacle to worldwide sustainable productivity and food security. Zinc sulfate (Zn) and paclobutrazol (PBZ) as a cost-effective agent, has multiple biochemical functions in plant productivity. Meanwhile, their synergistic effects on inducing salt tolerance are indecisive and not often reported. A pot experiment was done for evaluating the defensive function of Zn (100 mg/L) or PBZ (200 mg/L) on salt (0, 50, 100 mM NaCl) affected pea plant growth, photosynthetic pigment, ions, antioxidant capacity, and yield. Salinity stress significantly reduces all growth and yield attributes of pea plants relative to nonsalinized treatment. This reduction was accompanied by a decline in chlorophyll, nitrogen, phosphorus, and potassium (K+), the ratio between K+ and sodium (Na+), as well as reduced glutathione (GSH) and glutathione reductase (GR). Alternatively, salinity increased Na+, carotenoid (CAR), proline (PRO), ascorbic acid (AsA), superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX) over nonsalinized treatment. Foliar spraying with Zn and PBZ under normal condition increased plant growth, nitrogen, phosphorus, potassium, K+/Na+ ratio, CAR, PRO, AsA, GSH, APX, GR, and yield and its quality, meanwhile decreased Na+ over nonsprayed plants. Application of Zn and PBZ counteracted the harmful effects of salinity on pea plants, by upregulating the antioxidant system, ion homeostasis, and improving chlorophyll biosynthesis that induced plant growth and yield components. In conclusion, Zn plus PBZ application at 30 and 45 days from sowing offset the injuries of salinity on pea plant growth and yield by upregulating the antioxidant capacity and increasing photosynthetic pigments.In modern laying hybrids, calcium (Ca) homeostasis is immensely challenged by daily eggshell calcification. However, excessive mobilization of Ca from bones may lead to osteoporosis, which then manifests in a high incidence of poor bone quality. The aim of this study was to characterize the hens’ adaptation response to an alternating dietary Ca restriction. The animal model consisted of four purebred layer lines, differing in laying performance (high vs. moderately performing lines) and phylogenetic origin (white- vs. brown-egg lines). According to the resource allocation theory, hens selected for high egg production were assumed to show a different response pattern to cope with this nutritive challenge compared to moderately performing lines. Data collected included egg number, egg quality traits, body weight and bone characteristics. The Ca depletion led to a temporary drop in egg production and shell quality and a loss of bone stability due to Ca mobilization. The white-egg lines response was more pronounced, whereas the brown-egg lines were less sensitive towards reduced Ca supply. Our study shows that the hens’ responsiveness to coping with a nutritive Ca depletion is not ultimately linked to genetic selection for increased egg production but rather to phylogenetic origin.The cytokinesis-block micronucleus (CBMN) assay is a standardized method used for genotoxicity studies. Conventional whole blood cultures (WBC) are often used for this assay, although the assay can also be performed on isolated peripheral blood mononuclear cell (PBMC) cultures. However, the standardization of a protocol for the PBMC CBMN assay has not been investigated extensively. The aim of this study was to optimize a reliable CBMN assay protocol for fresh and cryopreserved peripheral blood mononuclear cells (PBMCS), and to compare micronuclei (MNi) results between WBC and PBMC cultures. The G0 CBMN assay was performed on whole blood, freshly isolated, and cryopreserved PBMCS from healthy human blood samples and five radiosensitive patient samples. Cells were exposed to 220 kV X-ray in vitro doses ranging from 0.5 to 2 Gy. The optimized PBMC CBMN assay showed adequate repeatability and small inter-individual variability. MNi values were significantly higher for WBC than for fresh PBMCS. Additionally, cryopreservation of PBMCS resulted in a significant increase of MNi values, while different cryopreservation times had no significant impact. In conclusion, our standardized CBMN assay on fresh and cryopreserved PBMCS can be used for genotoxicity studies, biological dosimetry, and radiosensitivity assessment.This paper presents a new solution enabling modeling of the mechanical stress tensor dependence of the 3D relative permeability tensor of isotropic material only on the basis of knowledge of the axial stress dependence characteristics. For the proposed model, the concept of principal stresses is utilized. In such a case, the sophisticated system of axial and shear stresses may be reduced to the set of axial stresses in a rotated coordination axes system. As a result, the proposed solution generalizes the explanation of the shape of magnetoelastic characteristics as well as radically extending possibility of the application of the finite elements methods (FEM) to describe sophisticated magnetoelastic systems.The large-scale preparation of stable graphene aqueous dispersion has been a challenge in the theoretical research and industrial applications of graphene. This study determined the suitable exfoliation agent for overcoming the van der Waals force between the layers of expanded graphite sheets using the liquid-phase exfoliation method on the basis of surface energy theory to prepare a single layer of graphene. To evenly and stably disperse graphene in pure water, the dispersants were selected based on Hansen solubility parameters, namely, hydrophilicity, heterocyclic structure and easy combinative features. The graphene exfoliation grade and the dispersion stability, number of layers and defect density in the dispersion were analysed under Tyndall phenomenon using volume sedimentation method, zeta potential analysis, scanning electron microscopy, Raman spectroscopy and atomic force microscopy characterization. Subsequently, the long-chain quaternary ammonium salt cationic surfactant octadecyltrimethylammonium chloride (0.3 wt.%) was electrolyzed in pure water to form ammonium ions, which promoted hydrogen bonding in the remaining oxygen-containing groups on the surface of the stripped graphene. Forming the electrostatic steric hindrance effect to achieve the stable dispersion of graphene in water can exfoliate a minimum of eight layers of graphene nanosheets; the average number of layers was less than 14. The 0.1 wt.% (sodium dodecylbenzene sulfonate melamine = 11) mixed system forms π-π interaction and hydrogen bonding with graphene in pure water, which allow the stable dispersion of graphene for 22 days without sedimentation. The findings can be beneficial for the large-scale preparation of waterborne graphene in industrial applications.

DNA mismatch repair (MMR) is a system for repairing errors in DNA replication. Cancer cells with MMR deficiency can have immunohistochemical loss of MMR protein expression leading to a hypermutable phenotype that may correlate with anti-PD1 efficacy. Scant data exist about immunohistochemical loss of MMR protein expression in high-grade gliomas (HGG).

We performed a large multicenter retrospective study to investigate the frequency and the prognostic role of immunohistochemical loss of MMR protein expression in HGG patients; we nevertheless evaluated the association between this status and clinical or molecular characteristics. Immunohistochemical loss of MMR protein expression was recorded as partial or complete loss of at least 1 MMR protein.

We analyzed the expression of MMR proteins in tumor tissue of 355 consecutive patients. Partial and complete immunohistochemical loss of MMR proteins was found in 43/355 samples (12.1%) and among these, 15 cases (4.2%) showed a complete loss of at the least one Mnhibitor efficacy in these subgroups of patients.In search for natural products with antimicrobial properties for use in the prevention and treatment of periodontitis, the purpose of this investigation was to evaluate the antimicrobial activity of two omega-3 fatty acids, docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), using an in vitro multi-species subgingival biofilm model including Streptococcus oralis, Actinomyces naeslundii, Veillonella parvula, Fusobacterium nucleatum, Porphyromonas gingivalis, and Aggregatibacter actinomycetemcomitans. The antimicrobial activities of EPA and DHA extracts (100 µM) and the respective controls were assessed on 72 h biofilms by their submersion onto discs for 60 s. Antimicrobial activity was evaluated by quantitative polymerase chain reaction (qPCR), confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM). ANOVA with Bonferroni correction was used to evaluate the antimicrobial activity of each of the fatty acids. Both DHA and EPA significantly reduced (p less then 0.001 in all cases) the bacterial strains used in this biofilm model. The results with CLSM were consistent with those reported with qPCR. Structural damage was evidenced by SEM in some of the observed bacteria. It was concluded that both DHA and EPA have significant antimicrobial activity against the six bacterial species included in this biofilm model.The interactions in model ionic YTX3···Z (Y = NC, F, Cl, Br; X = F, Cl, Br, Z = F-, Cl-, Br-, Li+) dyads containing the tetrel atoms, T = C, Si, Ge, were studied using ab initio computational methods, including an energy decomposition analysis, which found that the YTX3 molecules were stabilized by both anions (via tetrel bonding) and cations (via polarization). For the tetrel-bonded dyads, both the electrostatic and polarization forces make comparable contributions to the binding in the C-containing dyads, whereas, electrostatic forces are by far the largest contributor to the binding in the Si- and Ge-containing analogues. Model metastable Li+···NCTCl3···F- (T = C, Si, Ge) triads were found to be lower in energy than the combined energy of the Li+ + NCTCl3 + F- fragments. The pair energies and cooperative energies for these highly polar triads were also computed and discussed.In the current study, we analysed the role and prognostic value of myeloid-derived suppressor cells (MDSC) in chronic lymphocytic leukaemia (CLL). The frequency of circulating monocytic MDSC (M-MDSC; defined as CD14+CD11b+CD15-HLA-DR-/low cells) was assessed in correlation with clinical and laboratory parameters characterising the disease activity and patient immune status. Samples of peripheral blood from untreated CLL patients and healthy volunteers were stained with monoclonal antibodies for flow cytometry analysis. CLL patients with M-MDSC percentages above 9.35% (according to the receiver operating characteristic (ROC) analysis) had a shorter time-to-treatment and shorter survival time than the group with a lower percentage of M-MDSC. The M-MDSC percentage was higher in patients with adverse prognostic factors (i.e., 17p and 11q deletion and CD38 and ZAP-70 expression). A high M-MDSC percentage was linked to significantly lower expression of the CD3ζ in T cells. Furthermore, an analysis of immune regulatory molecules (arginase 1 (ARG1), nitric oxide synthase (NOS2), indoleamine 2,3-dioxygenase (IDO), transforming growth factor beta (TGF-β), and interleukin (IL)-10) was performed. By the means of flow cytometry and RT-qPCR, we showed an overexpression of three of them in M-MDSC of CLL patients. M-MDSC cells seem to be an important factor in the immunosuppressive microenvironment of CLL and seem to be a good and novel prognostic factor.Climate change has become a controversial topic in today’s media despite decades of warnings from climate scientists and has influenced human health significantly with the increasing prevalence of infectious pathogens and contribution to antimicrobial resistance. Elevated temperatures lead to rising sea and carbon dioxide levels, changing environments and interactions between humans and other species. These changes have led to the emergence and reemergence of infectious pathogens that have already developed significant antimicrobial resistance. Although these new infectious pathogens are alarming, we can still reduce the burden of infectious diseases in the era of climate change if we focus on One Health strategies. This approach aims at the simultaneous protection of humans, animals and environment from climate change and antimicrobial impacts. Once these relationships are better understood, these models can be created, but the support of our legislative and health system partnerships are critical to helping with strengthening education and awareness.In this study, we visualised CO2-brine, density-driven convection in a Hele-Shaw cell. Several experiments were conducted to analyse the effects of the salinity and temperature. The salinity and temperature of fluids were selected according to the storage site. By using charge coupled device (CCD) technology, convection finger formation and development were obtained through direct imaging and processing. The process can be divided into three stages diffusion-dominated, convection-dominated and shutdown stages. Fingers were formed along the boundary at the onset time, reflecting the startup of convection mixing. Fingers formed, moved and aggregated with adjacent fingers during the convection-dominated stage. The relative migration of brine-saturated CO2 and brine enhanced the mass transfer. The effects of salinity and temperature on finger formation, number, and migration were analysed. Increasing the salinity accelerated finger formation but suppressed finger movement, and the onset time was inversely related to the salinity. However, the effect of temperature on convection is complex. The dissolved CO2 mass was investigated by calculating the CO2 mass fraction in brine during convection mixing. The results show that convection mixing greatly enhanced mass transfer. The study has implications for predicting the CO2 dissolution trapping time and accumulation for the geological storage of CO2.The activity-dependent neuroprotective protein (ADNP), a double-edged sword, sex-dependently regulates multiple genes and was previously associated with the control of early muscle development and aging. Here we aimed to decipher the involvement of ADNP in versatile muscle gene expression patterns in correlation with motor function throughout life. Using quantitative RT-PCR we showed that Adnp+/- heterozygous deficiency in mice resulted in aberrant gastrocnemius (GC) muscle, tongue and bladder gene expression, which was corrected by the Adnp snippet, drug candidate, NAP (CP201). A significant sexual dichotomy was discovered, coupled to muscle and age-specific gene regulation. As such, Adnp was shown to regulate myosin light chain (Myl) in the gastrocnemius (GC) muscle, the language acquisition gene forkhead box protein P2 (Foxp2) in the tongue and the pituitary-adenylate cyclase activating polypeptide (PACAP) receptor PAC1 mRNA (Adcyap1r1) in the bladder, with PACAP linked to bladder function. A tight age regulation was observed, coupled to an extensive correlation to muscle function (gait analysis), placing ADNP as a muscle-regulating gene/protein.Few studies have focused on quantifying the double burden of malnutrition (DBM) phenomenon in China. We aimed to clarify the prevalence of DBM among Chinese adults as well as to examine whether usual daily dietary micronutrient status varies by body mass index (BMI) categories. In this study, a sample of 6602 adults aged 18-59 years from the China Health and Nutrition Survey (CHNS) was analyzed. Information was obtained on dietary intake and anthropometric measurements. Dietary intakes of 11 micronutrients were estimated based on the data collected by three consecutive days of 24 h recalls combined with the weighing of household seasonings. Dietary micronutrient deficiency was defined according to the cutoff of the Chinese estimated average requirement (EARs). 44% of Chinese adults faced the problem of DBM, of which nearly 40% experienced overweight/obesity and micronutrient deficiency simultaneously. Comparable percentages (>50%) of Chinese adults had dietary intake less than the Chinese EARs for key micronutrients including retinol, thiamin, riboflavin, vitamin C, calcium, selenium, zinc, and magnesium, and the percentages varied by body weight status. More than 80% participants had at least two selected vitamin or mineral deficiencies in all BMI categories. These findings indicate that Chinese adults have a high DBM and micronutrient inadequacies prevail among and within gender and all BMI categories. All body weight groups need advice on the changing needs for dietary variety to ensure optimal health.Microwave pretreatment of oilseeds is a novel technique used to enhance oil nutraceutical properties. In this study, the effect of microwave pretreatment of seeds was investigated on pomegranate seed oil quality attributes including oil yield, yellowness index, refractive index, peroxide value, ρ-anisidine value, total oxidation value, conjugated dienes, total phenolic content, total carotenoids content, phytosterol composition, fatty acid composition, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity, and ferric reducing antioxidant power (FRAP). The seeds of three different pomegranate cultivars (’Acco’, 'Herskawitz’, and 'Wonderful’) were microwave heated at 261 W for 102 s. Pomegranate seeds microwave pretreatment enhanced oil yield, yellowness index, total carotenoids content, total phenolic content, FRAP and DPPH radical scavenging capacity, despite an increase in conjugated dienes, and peroxide value. Palmitic acid, oleic acid, linoleic acid, saturated, and monosaturated fatty acids were increased after pomegranate seeds microwave pretreatment, whilst the levels of punicic acid and β-sitosterol were reduced. Nevertheless, the refractive index, the ratio of unsaturated to saturated fatty acid of the extracted oil were not significantly (p > 0.05) affected by pomegranate seeds microwave pretreatment. Principal component analysis and agglomerative hierarchical clustering established that 'Acco’ and 'Wonderful’ oil extracts from microwave pretreated PS exhibited better oil yield, whilst 'Herskawitz’ oil extracts showed higher total carotenoids content, total phenolic content, and antioxidant capacity.

Prognosis of oral squamous cell carcinoma (OSCC) is difficult to exactly assess on pre-operative biopsies. Since OSCC DNA methylation profile has proved to be a useful pre-operative diagnostic tool, the aim of the present study was to evaluate the prognostic impact of DNA methylation profile to discriminate OSCC with high and low aggressive potential.

36 OSCC cases underwent neoplastic cells collection by gentle brushing of the lesion, before performing a pre-operative biopsy. The CpG islands methylation status of 13 gene (

,

,

,

,

,

,

,

,

,

,

,

,

) was studied by bisulfite Next Generation Sequencing (NGS). A Cox proportional hazards model via likelihood-based component-wise boosting was used to evaluate the prognostic power of the CpG sites.

The boosting estimation identified five CpGs with prognostic significance

,

,

,

and

. The combination of significant

provided promising results for adverse events prediction (Brier score = 0.080, C-index = 0.802 and AUC = 0.850).

had a strong prognostic power in patients with early OSCC.

These data confirm that the study of methylation profile provides new insights into the molecular mechanisms of OSCC and can allow a better OSCC prognostic stratification even before surgery.

These data confirm that the study of methylation profile provides new insights into the molecular mechanisms of OSCC and can allow a better OSCC prognostic stratification even before surgery.A series of BiVO4 photocatalysts were prepared by a co-precipitation followed hydrothermal synthesis method for the photodegradation of Rhodamine B (RhB) and 2,4-Dichlorophenol (2,4-DCP). The crystalline phase ratio of the heterostructured BiVO4 (m-BiVO4/t-BiVO4) between monoclinic and tetragonal could be easily controlled at different pH and hydrothermal time. Interestingly, the as-prepared heterostructured BiVO4 photocatalyst at pH = 7 for 24 h (BiVO4-7-24) showed the highest photocatalytic activities for the degradation of RhB, while the best photodegradation of 2,4-DCP was obtained at pH = 0.5 for 24 h (BiVO4-0.5-24). The photocatalytic mechanism can be explained by the different charge carrier transfer pathways and active oxidation species in the m-BiVO4/t-BiVO4 heterostructure. More importantly, the exposed facets originated from crystalline phase controlling in BiVO4-0.5-24 and BiVO4-7-24 photocatalyst is an essential reason for the different photocatalytic activity. The proposed energy band alignments of BiVO4-0.5-24 and BiVO4-7-24 photocatalyst provide insights into the photocatalytic mechanism of the m-BiVO4/t-BiVO4 heterostructure.Traditional enzyme-linked immunosorbent assay (ELISA) suffers from the limitations of relatively low sensitivity and stability, and enzyme-labelled antibodies are hard to be prepared and purified. Based on a nanozyme, an aptamer and Fe3O4 magnetic nanoparticles (MNP), a nanozyme and aptamer-based immunosorbent assay (NAISA) was developed for aflatoxin B1 (AFB1) detection with simpler operation and separation. In this work, mesoporous SiO2/Au-Pt (m-SAP) were prepared to act as signal labels, which showed high catalase-like activity and was denoted as nanozyme. Aptamer was adopted to specifically recognize with AFB1, and MNP facilitated to realize magnetic separation. To verify the performance of NAISA, traditional ELISA (t-ELISA) and enhanced ELISA (e-ELISA) using MNP and m-SAP nanozyme were applied in AFB1 detection. The NAISA method showed the lowest limit of detection (LOD) with 5 pg mL-1 (n = 3, ±4.2 %), 600 and 12-fold lower than that of t-ELISA (3 ng mL-1) and e-ELISA (0.06 ng mL-1), respectively. In the interference tests, AFB1 can be identified among six different interfering substances. The NAISA method, thus, can be of great importance as it allows selective and sensitive AFB1 detection, while providing the simplicity of use and need for screening hazardous materials.The acoustic parameters and operating conditions that determine efficiency of oil recovery from oily sludge are studied. Based on this, the mechanism of ultrasonic disintegration of oily sludge is analyzed. The results show that lower frequency ultrasound results in larger and more energetic cavitation bubbles that are more effective in the desorption of oil from solid particles. Moreover, acoustic intensity and treatment time that correspond to maximal oil recovery are found. Increasing the ratio of water to sludge and pH can reduce the slurry viscosity and facilitate the formation of HSiO3-, respectively, which improves the oil recovery efficiency. Moreover, Triton X-100 has better oil solubilizing effects than SDBS. After ultrasonic treatment, small amounts of asphaltenes are more stable on solid particles than other components. The heteroatoms such as S, N, and O in asphaltenes form hydrogen bonds with hydroxyl groups on the surface of the particles, impeding the desorption of oil. Mechanical effects such as shock waves and micro jets due to acoustic cavitation can break the hydrogen bonds between asphaltenes and solid particles, thereby facilitating oil recovery from oily sludge.Organo-bentonite (OrB) was prepared by modifying bentonite with chitosan, and natural surfactant extracted from Sapindus rarak fruit. The physical alteration post-modification, performance of phosphates (Pi) adsorption, and possibility as a Pi-supplementation for plants of OrB were assessed and compared to acid-activated bentonite (AAB). The physical alteration due to modification of bentonite was characterized. SEM images were not indicating significant morphology differences between OrB and AAB. Existence of chitosan layers in OrB causes a decrease in basal spacing as characterized using XRD. The BET surface area of OrB was decreased compared to AAB due to pore coverage by chitosan. Adsorption studies reveal that OrB has a higher adsorption capacity towards Pi than AAB, which is 97.608 and 131.685 mg/g at 323 K for AAB and OrB, respectively. The H-shape isotherm curve indicates that chemisorption is dominantly controlling the adsorption. The isotherm and kinetics adsorption were well fitted to Langmuir and Pseudo-second order models, respectively. Performance of AAB and OrB as Pi-supplementation was assessed based on growth phenotypes of Arabidopsis thaliana; seedlings show that supplementation of Pi@AAB and Pi@OrB (at half doses) can promote primary root extension. These results also demonstrate the safety of direct disposal of the materials into the soil.In this work, the biodurability of three silica particle types (synthetic amourphous silica, MCM-41 microparticles, MCM-41 nanoparticles) functionalised with three different essential oil components (carvacrol, eugenol, vanillin) was studied under conditions that represented the human gastrointestinal tract and lysosomal fluid. The effect of particle type, surface immobilised component and mass quantity on the physico-chemical properties of particles and silicon dissolution was determined. Exposure to biological fluids did not bring about changes in the zeta potential values or particle size distribution of the bare or functionalised materials, but the in vitro digestion process partially degraded the structure of the MCM-41 nanoparticles. Functionalisation preserved the structure of the MCM-41 nanoparticles after simulating an in vitro digestion process, and significantly decreased the amount of silicon dissolved after exposing different particles to both physiological conditions, independently of the essential oil component anchored to their surface. The MCM-41 microparticles showed the highest solubility, while synthetic amorphous silica presented the lowest levels of dissolved silicon. The study of these modified silica particles under physiological conditions could help to predict the toxicological behaviour of these new materials.Recently, simultaneous sulfide removal and bioenergy production by microalgal treatment have attracted growing attention. However, the response of nitrogen metabolism to the sulfide-removal process has yet to be explored. Here, variable levels of sulfide could be completely removed by Chlamydomonas sp. Tai-03 under both high and low nitrate conditions in synthetic wastewaters. The highest sulfide removal rate of 5.56 mg-S L-1 h-1 was achieved with the addition of 100 mg L-1 sulfide in the presence of high nitrate. Meanwhile, sulfide was chemically oxidized to sulfate and then ingested by microalgae. Interestingly, sulfide-removal efficiency critically depended on nitrate concentration. Sulfide can also enhance the ability of microalgae to assimilate nitrogen. Based on the analysis of sulfur- and nitrogen-related metabolic profiling, serine as a precursor decreased by 94 % under low levels of nitrate, which induced the significant inhibition of cysteine and methionine biosynthesis. The results indicated that nitrogen source played a critical role in the sulfur cycle because of the positive relationship between the aforementioned metabolic processes and nitrate concentration. Additionally, sulfide can improve lipid and carbohydrate productivity under high levels of nitrate. This study enhances our understanding of the mechanisms underlying the simultaneous removal of sulfide and alternative bioenergy production.p-n Heterojunctions of BiOI/ZnO nanorod arrays (BiOI/ZnO NRs) were prepared by loading the p-type BiOI nanosheets on the n-type ZnO nanorod arrays for efficient removal of organic contaminants in water during the piezo-photocatalytic degradation. Under concurrent visible-light irradiation and ultrasonic vibration, the bisphenol solution (50 mL, 10 mg/L) could be completely degraded within 30 min by 10 mg of 0.15 BiOI/ZnO NRs. It shows a dramatically-enhanced degradation efficiency under light irradiation and ultrasonic vibration, which is four times as high as that only under light irradiation. The excellent piezo-photocatalytic ability of BiOI/ZnO NRs could be attributed to the piezoelectric effect coupling with photocatalytic process. Under the irradiation of light, the electron-hole pairs were generated in BiOI nanosheets, and the piezoelectric potential is created inside the highly oriented one-dimensional ZnO nanorods by ultrasonic vibration, which can accelerate the migration of photogenerated carriers. It shows a strategy to effectively enhance the photocatalytic activity through utilizing the internal piezoelectric potential, which is generated by the one-dimensional nanorods with piezoelectric properties under ultrasonic vibration. So, it can promote the separation and prolong the lifetime of photogenerated carriers, and result in high-efficient degradation of organic contaminants.Bamboo has been considered a potential plant species for phytoremediation due to its high biomass and heavy metal (HM) resistance. However, little is known about the interactions between bamboo and soil microbial activities in HM-contaminated soils. Here, we investigated the characteristics of microbial communities in the rhizosphere soil of Lei bamboo (Phyllostachys praecox) along a chromium (Cr) gradient. We found that the soil Cr content was positively correlated with the total organic carbon (TOC) and HCl-extractable Cr but negatively correlated with the pH and bacterial and fungal Shannon indices. Proteobacteria and Ascomycota predominated in the bamboo rhizosphere under Cr pollution. A co-occurrence network showed that two of the most Cr-sensitive bacterial genera and keystone taxa were from the Acidobacteria, indicating that this phylum can be as an indicator for the studied Cr-polluted soils. Redundancy analysis revealed that both the soil bacterial and fungal community compositions were significantly correlated (p less then 0.05) with Cr, pH, TOC, alkali-hydrolysable N (AN), and available phosphorus (AP). The increase in TOC as the Cr content increased can be ascribed to an adverse Cr effect on the soil microflora, probably because the microbial biomass was less effective in mineralizing soil C under Cr-polluted conditions.Expanded graphite (EG) immobilized nickel ferrite (NiCo2O4) was successfully constructed by a simple hydrothermal approach and applied for the degradation of sulfamethoxazole (SMX) in model wastewater by peroxymonosulfate (PMS) activation. The features of prepared catalysts were characterized by SEM, TEM, EDS, XRD, BET, TPD and XPS techniques. The influences of several critical parameters including the prepared NiCo2O4-EG dosages, PMS concentrations, temperature, initial solution pH and inorganic ions on SMX removal were studied in details. In particular, the synthesized NiCo2O4-EG exhibits excellent catalytic performances for SMX depredation over a wide pH range (pH 3.0-11.0). Besides, the transformation of various reactive oxygen species (SO4-, HO, O2- and 1O2) with the change of initial pH was investigated by the electron paramagnetic resonance (EPR) and quenching tests. In addition, twelve major degradation intermediates of SMX were detected by UPLC-QTOF-MS/MS. Finally, the PMS activation mechanism in NiCo2O4-EG/PMS system by the synergistic coupling of EG and NiCo2O4 were put forward. In brief, this work provided a promising and potential catalyst for PMS activation to remove SMX from wastewater.To study the inhibited degradation metabolism and anaerobic digestion of typical lipids in food waste, an artificially produced capsaicin, N-Vanillylnonanamide, a typical soluble component in waste lipids, was added to a glycerol trioleate anaerobic digestion system. The microorganisms damage and blocked electron transfer caused by N-Vanillylnonanamide during anaerobic digestion were further clarified. Scanning electron microscopy and transmission electron microscopy images demonstrated that N-Vanillylnonanamide (≥4 wt%) structurally damaged microorganisms via cell membrane breakage, which impair their function. N-Vanillylnonanamide inhibited the activities of the key enzyme CoA, AK, F420, and CoM, which are relevant for both degradation metabolism and anaerobic digestion. 16S rRNA analysis showed that dominant bacterial and archaeal communities markedly decreased after anaerobic digestion of glycerol trioleate with N-Vanillylnonanamide (≥4 wt%). For example, the proportion of Methanosarcina decreased from 30 % to 6 %. Current-voltage curves indicated that the electron transfer rate in the community of microorganisms decreased by 99 % from 4.67 × 10-2 to 5.66 × 10-4 s-1 in response to N-Vanillylnonanamide (40 wt%). The methane yield during anaerobic digestion of glycerol trioleate decreased by 84.0 % from 780.21-142.10 mL/g-total volatile solids with N-Vanillylnonanamide (40 wt%).An effective strategy for enhancement of catalytic activity and stability of immobilized laccase via metal affinity adsorption on Fe3O4@C-Cu2+ nanoparticles was developed, which involved the fabrication of hydroxyl and carboxyl functionalized Fe3O4@C nanoparticles via a simple hydrothermal process and the subsequent chelation with Cu2+ for the immobilization of laccase under a mild condition. Our results revealed that the Fe3O4@C-Cu2+ nanoparticles possess a high loading amount of bovine serum albumin (BSA, 436 mg/g support) and laccase activity recovery of 82.3 % after immobilization. Laccase activity assays indicated that thermal and pH stabilities, and resistances to organic solvents and metal ions of the immobilized laccase were relatively higher than those of the free enzyme. The immobilized laccase maintained more than 61 % of its original activity after 10 consecutive reuses. Most importantly, the immobilized laccase possessed excellent degradation of diverse synthetic dyes. The degradation rates of malachite green (MG), brilliant green (BG), crystal violet (CV), azophloxine, Procion red MX-5B, and reactive blue 19 (RB19) was approximately 99, 93, 79, 88, 75 and 81 (%) in the first cycle. Even after 10 consecutive reuses, the removal efficiencies of the six dyes were found to be 94, 80, 71, 78, 60, and 65 (%), respectively.Elimination of U(VI) from polluted solutions is important for human health and environmental safety. In this work, a relatively low-cost 3D flower-like phosphate-functionalized layered double hydroxides (phos-LDH) was fabricated by a one-pot hydrothermal method. The prepared phos-LDH inherited the structure of 3D flower-like layered double hydroxides (LDH), and had a higher specific surface area (∼203.4 m2⋅g-1) than that of LDH. The kinetic process indicated that U(VI) adsorption onto phos-LDH achieved equilibrium within 15 min and obeyed general order model. The adsorption isotherms of phos-LDH illustrated that the U(VI) adsorption obeyed Langmuir model, the adsorption capability of phos-LDH can reach 923.1 mg⋅g-1 at 298 K. The U(VI) adsorption was a spontaneous and endothermic process according to the thermodynamic data. There was the electrostatic attraction between U(VI) and phos-LDH at pH = 5.0. FTIR and XPS analyses educed that the hydroxyl and phosphate groups played a very useful role for the complexation between U(VI) and phos-LDH. In addition, the excellent selective adsorption capability for U(VI) in competitive cation and anion solutions further confirmed the practical application of phos-LDH in real wastewater treatment.The novel combined system using Na2S2O8/urea was used to simultaneously absorb nitric oxide and sulfur dioxide emissions from marine diesel engines as well as inhibit the formation of nitrate in cleaning wastewater to meet the increasingly stringent requirements of regulations. The influences of reaction temperature, Na2S2O8 concentration, urea concentration, SO2 concentration, NO concentration and pH value on SO2 removal efficiency, NO removal efficiency and nitrate concentration were investigated. The experimental results showed that different reaction temperatures had different influences on SO2 removal efficiency, NO removal efficiency and nitrate concentration. An increase in Na2S2O8 could improve the absorption of NO. The addition of urea could effectively improve the removal efficiency of NO and reduce the nitrate concentration. The removal efficiencies of 1000 ppm NO and 1000 ppm SO2 achieved 100 % with 0.2 mol/L Na2S2O8 and 2 mol/L urea at 70℃, and the nitrate content was 8.56 mg/L which was far lower than the regulatory requirement of 60 mg/L. The acidic condition (pH ≤ 5.5) not only facilitated the absorption of NO but also reduced the generation of nitrate. According to the experimental results, the novel combined system was promising to be applied to the control technology of marine diesel engine exhaust.The value-added utilization of waste resources to synthesize functional materials is important to achieve the environmentally sustainable development. In this work, novel micro-nano FeOx- and MnOx-modified bone biochars derived from waste bone meal were obtained at 300 °C, 450 °C and 600 °C, and applied to remove Cd(II), Cu(II) and Pb(II) from aqueous solutions. The results showed that the pyrolysis temperature greatly influenced the specific surface area (SSA), micropore creation, functional groups and heavy metal sorption capacities of FO-BCs and MO-BCs. The effects of solution pH, ionic strength, humic acid (HA), kinetics and thermodynamics on heavy metals adsorption were investigated. Langmuir and pseudo-second order kinetics models fit the adsorption data well, and the FO-BC-450 and MO-BC-600 displayed the highest sorption capacity for Cd(II) (151.3 mg/g and 163.4 mg/g), Cu(II) (219.8 mg/g and 259.0 mg/g) and Pb(II) (271.9 mg/g and 407.2 mg/g), respectively. Due to the dissolved partial hydroxyapatite (HAP), carbonate-bearing hydroxyapatite (CHAP) and the catalysis of Fe(NO3)3, the FO-BCs with higher SSA than the MO-BCs, whereas the sorption capacity displayed an opposite trend. The chemical complex, cation-π bonds, ion exchange and coprecipitation were the dominant mechanisms for metals adsorption. Overall, waste bone resource co-pyrolysis with Fe(NO3)3/KMnO4 impregnation is a promising and high-efficient adsorbents for the remediation of heavy metals-contaminated waters.Heavy metal pollution caused by stormwater runoff has triggered a demand for effective heavy metal sorbents. Effective heavy metal removal using conventional stormwater runoff treatment processes that employ filtration mechanisms as primary removal mechanisms is difficult. Therefore, we attempt to improve cadmium removal performance by attaching disulfide polymer (DiS-COP) containing soft bases, thiols, onto the surface of polypropylene/polyethylene (PP/PE) fiber media, which is widely used for stormwater runoff treatment. Material characterization demonstrated that DiS-COP was successfully grafted and grown on the surface of PP/PE (Dis-PP/PE). The batch and continuous flow adsorption capacities of Dis-PP/PE were 81.1 mg/g and 2.33 mg/g, respectively, which is 40 times higher than those of pristine PP/PE. Applicability of DiS-PP/PE at pH 6-8 was demonstrated, and effects of calcium and humic acid on cadmium adsorption were investigated. Calcium marginally affected cadmium adsorption, which can be explained using the Hard and soft (Lewis) acids and bases theory (HSAB), but cadmium removal efficiency decreased owing to humic acid (HA)-Cd complex formation and agglomeration in the presence of organic material. In a breakthrough test, the adsorption column exhibited complete cadmium uptake over 24 h until it reached the breakthrough point. Therefore, heavy metal adsorption performance of PP/PE was successfully enhanced by grafting DiS-COP on its surface.Formaldehyde is a carcinogenic indoor air pollutant emitted from wood-based furniture, building materials, paints and textiles. Yet, no low-cost sensor exists for on-site monitoring to fulfill stringent current and upcoming (e.g., 8 parts-per-billion by volume, ppb, in France by 2023) exposure guidelines. Here, we present an inexpensive and handheld formaldehyde detector with proven performance in real indoor air. Selectivity is achieved by a compact packed bed column of nanoporous polymer sorbent that separates formaldehyde from interferants present in ambient air. Downstream, a highly sensitive nanoparticle-based chemoresistive Pd-doped SnO2 sensor detects formaldehyde in the relevant concentration range down to 5 ppb within 2 min. As a proof-of-concept, we measured formaldehyde in indoor air and from different wood product emissions, in excellent agreement (R2 > 0.98) with high-resolution proton-transfer-reaction time-of-flight mass spectrometry. This detector is simple-in-use and readily applicable for on-site formaldehyde exposure monitoring at home or work. It is promising for internet-of-things (IOT) sensing networks or even wearables for personal exposure assessment.Xuan-paper waste residue (XPWR) is an unfamiliar and unique solid waste in China, which caused serious environmental pollution and waste of resources. Therefore, it is extremely important to explore the characteristics of XPWR and its application. In this paper, XPWR was analyzed and used as a reinforcing filler to prepare red mud/waste polyethylene/Xuan-paper waste residue (RM/WPE/XPWR) composites by molding method at 160 °C with a pressure of 10 MPa for 5-10 min. There were about 27 wt% of mineral particles and 63 wt% of organic fibers in XPWR, indicating that XPWR can be used as a reinforcing filler. When 60 wt% of XPWR was added in RM/WPE, the bending strength reached 71.81 MPa, which surpassed 43.08 % than that of RM/WPE. Besides, the addition of XPWR increased the water absorption of the composites and helped to promote the crystallization of the composites. This work presented the characteristics of XPWR and provided a new way to use XPWR.To recover the spent vanadium compound, Rhodamine-B-based Schiff’s base ligand (L1) was synthesized via ultrasonication process and was evaluated with vanadyl sulfate (VOSO4), which has shown considerable selectivity towards V(IV). The change of the solution color from colorless to pink is attributed to L1 after the reaction with vanadium ion owing to the successful formation of the vanadium complex and the opening of the spirolactam ring in the L1 structure. In FT-IR spectra, the vanadyl peaks are co-existed with the L1 structure, which confirmed the complex formation of the L1 with vanadium. Similarly, the binding energy of V(IV) was identified at 516.2 eV for V2p3/2 in XPS spectra. The new strategy for VOSO4 recovery was established through solvent extraction and acid leaching. After recovery process, the absence of vanadium peak in the XPS confirmed the complete removal of V(IV) from the complex. The recovered VOSO4 solution used as an electrolyte in vanadium redox flow battery (VRFB) systems, where the unit cell performance is comparable with the conventional electrolyte solution. The advantage of study is reuse of VOSO4 as a resource for energy storage applications.In this study, magnetic material based reduced graphene oxide (M-rGO) was prepared through co-precipitation and displayed high catalytic efficiency together with persulfate (PS) for simultaneous p-arsanilic acid (p-ASA) decomposition and arsenic removal. Linear sweep voltammetry and chronoamperometric measurements with M-rGO revealed that PS was effectively bound to M-rGO surface and probably formed charge transfer complex, in which M-rGO was pivotal in mediating facile electron transfer. The effects of pH, temperatures, anions, p-ASA concentration, PS, and M-rGO dosages on p-ASA decomposition were studied in the system. Excellent degradation of p-ASA was carried out at a wide range of pH values, which was unattainable by other Fenton-like processes. Under optimal conditions, M-rGO exhibited prominent removal of both p-ASA (98.8 %) and inorganic arsenic (89.8 %). M-rGO had reasonably excellent repeatability and stability, and 77.7 % p-ASA degraded in the third recovered catalyst. The advantages of environmental friendliness, short reaction time, and straightforward synthesis of M-rGO will facilitate the development of heterogeneous Fenton-like catalysts under neutral conditions.Trace element contamination from abandoned mine sites is a major threat to the environment. The distribution of trace elements in various particle size fractions of soils from abandoned mine sites plays a critical role in designing remediation approaches. This study investigated the geochemical distribution of trace element enrichment and mineralogical composition in various particle size fractions from contrasting abandoned mine sites (Webbs Consols, Halls Peak and Mole River, Australia). Results revealed that arsenic and other element concentrations increased with decreasing particle size for samples from Webbs Consols and Halls Peak. The highest arsenic (3.05%), lead (3.23%) and zinc (1110 mg/kg) were found in the finest fraction ( less then 0.053 mm). In Mole River, the highest concentration of arsenic (10.8%), lead (209 mg/kg) and zinc (351 mg/kg) were observed in coarse fractions. Arsenic fractionation by sequential extraction showed that arsenic was strongly associated with the amorphous and crystalline iron phases. X-ray diffraction (XRD) and scanning electron microscopy (SEM) studies revealed that tooeleite (a ferric arsenite mineral, also confirmed by Transmission electron microscopy (TEM)), arsenopyrite, scorodite and arsenolite were the dominant arsenic minerals. The study showed elevated levels of arsenic bearing minerals across particle sizes which has significant implications for remediation approaches at abandoned mine sites.