These results indicated that the LBG-SA coatings with 0.32 mg·mL-1 daphnetin were a potential alternative way to improve the quality of turbot during refrigerated storage.Advanced glycation endproducts (AGEs) are the final product of glycation, highly reactive in nature and contribute directly or indirectly to numerous complications related to diabetes. In this study, the antiglycation activity of glyburide was investigated using HSA as model protein, both against glucose and methylglyoxal mediated glycation. The possible mechanism of action was also deciphered using biophysical and computational tools. Approximately 70% inhibition of both early and advanced glycation end products were recorded in the presence of glyburide. Free lysine modification was reduced by glyburide treatment and improvement in biochemical markers such as free thiol groups and carbonyl content was observed. Interaction studies revealed that glyburide showed moderate to strong binding affinity towards HSA with binding constant in the order of 106 M-1. The interaction of glyburide with HSA was entropically favourable and spontaneous in nature. Molecular dynamics simulation deciphered that glyburide-HSA complex was quite stable where RMSD, RMSF, Rg, SASA, and secondary structure of HSA remained approximately same over the entire simulation period. The average binding energy of the MD simulation for glyburide-HSA complex was found to be -15.386 kJ mol-1. The findings demonstrate the antiglycation potential of glyburide and its possible mechanism of action.Phospholipases D (PLDs) are phospholipid hydrolyzing enzymes and crucial components of lipid signaling in plants. PLDs are implicated in stress responses in different plants however, characterization of PLDs in chickpea is missing. Here, we identify 13 PLD genes in the chickpea genome. PLD family could be divided into α, β, δ, ε and ζ isoforms based on sequence and structure. Protein remodeling described that chickpea PLDs are composed of defined arrangements of α-helix, β-sheets and short loops. Phylogenetic analysis suggested evolutionary conservation of chickpea PLD family with dicots. In-planta subcellular localization showed the plasma membrane localization of chickpea PLDs. All PLD promoters had hormone and stress related cis-regulatory elements, which suggested overlapping function of PLDs in hormone and abiotic stress signaling. The qRT-PCR expression analysis revealed that most PLD genes are differentially expressed in multiple abiotic stresses (drought, salt and cold stress). Moreover, several PLD genes had overlapping expression in abiotic stress and ABA and JA treatment. These observations indicate the involvement of PLD gene family in cross-talk of phytohormone and abiotic stress signaling in chickpea. Thus, present study opens new avenues of utilizing PLD related information for understanding hormone-regulated abiotic stress signaling in legume crops.Detection of metal catalyzed carbonylation in proteins is traditionally based on derivatization followed by detection and quantification via spectroscopy or immunodetection. However, these measure only cumulative carbonylation and do not identify the specific sites of modification within the protein. Recently, fluorescein thiosemicarbazide (FTC) based semi-microplate method was adapted for high throughput monitoring of carbonyl content during mAb process development, using size-exclusion chromatography followed by ultraviolet and fluorescence detection. Here, we have examined carbonylation in originators and 4 biosimilars of an IgG1 therapeutic monoclonal antibody, trastuzumab, a first line of therapy for HER2 positive breast cancer. The hyphenated RP-ESI-MS/MS detection was able to identify the location of each of the carbonylated amino acids for all products. The result is a comprehensive map of a total of 27 unique identified carbonylation sites of trastuzumab found across multiple batches of originator as well as marketed biosimilars. Our results demonstrate that although the different carbonylation sites are spread across different domains throughout the mAb sequence, the complementarity determining regions (CDRs) are free of carbonylation and all identified sites lie within the framework region of the variable domain. Moreover, the constant- heavy domain 3 (CH3) region seems to be particularly resistant to process induced carbonylation.In this research, the adsorption of three synthetic dyes dissolved in an aqueous solution on chitosan cryogel beads (Q-C-EGDE) was compared. The effect of the pH of the solution on the adsorption capacity of each dyes was analyzed. Furthermore, the kinetics and adsorption isotherms were compared, at temperatures of 283.15 K, 303.15 K and 323.15 K, and the kinetic and adsorption equilibrium data were fitted to three mathematical models, respectively. The biosorbent was characterized by scanning electron microscopy (SEM), the nitrogen physisorption BET method and Fourier transform infrared spectroscopy (FTIR). The characterization results show that the cryogel is composed of low-surface, macroporous, porous grooved walls. The functional groups that took part in the adsorption were mainly amino groups (NH3+). When comparing the adsorption capacities, it was found that the dyes adsorb in the following order Blue 1 > Red 2 > Yellow 5 reaching capacities from 1600 mg/L to 850 mg/L. The results of the adsorption and mathematical modelling suggest that the process is regulated mainly by physisorption and is largely limited by mass transfer mechanisms within the cryogel, where the electrostatic charges present affect adsorption. The latter was corroborated by the Monte Carlo simulation.The large agglomeration of starch paste in hot water, and fast retrogradation tendency and low transparency of starch gel restrict widespread application of kudzu starch. To improve the above defects, kudzu starch was modified with sequentially α-amylase (AA) and transglucosidase (TG), the latter for varying times. The results indicated that, compared to kudzu starch, amylose content and molecular weight of AA/TG-treated starches reduced by 20.07% and 69.50%, respectively. The proportion of A chain increased by 68.68%, whereas B1, B2 and B3 chains decreased by 14.28%, 48.29% and 23.44%, respectively. The degree of branching dramatically increased by 128.3%. After AA→TG treatment, the changes of starch structure enhanced the functional properties of kudzu starch. The solubility, paste clarity and gelatinization temperature increased, whereas the relative crystallinity, viscosity, storage and loss moduli decreased. Overall, the AA→TG modification would be desirable to improve the functional properties of kudzu starch to expand more large-scale application.In this study, bi-component alginate-hyaluronic acid (AHA) fibers were developed by using two different routes. In the first method, sodium alginate dope solution was extruded into a coagulation bath containing CaCl2 and subsequently dip-coated with hyaluronic acid (HA) whereas, in the second method, hyaluronic acid-containing sodium alginate dope solution was directly extruded into CaCl2 bath. The resulting AHA fibers were then dehydrated in 25-100% v/v acetone solutions and dried in air. The fibers were characterized by surface morphology, physicochemical analysis, mechanical performance, swelling percentage, and total liquid absorption (g/g), cell viability, and release behavior. The results showed that AHA fibers produced by the second method have better mechanical performance, high liquid absorption, and swelling percentage with a more controlled release of hyaluronic acid. The AHA fibers showed high biocompatibility toward nHDF cell line in in-vitro testing, and the MVTR values (650-800 g/m2/day) are in a suitable range for maintaining a moist wound surface proving to be appropriate for promoting wound healing.To study dextran degradation by sonoenzymolysis, the degradation rate, the change of molecular weight, the mass fractions of fragments of certain molecular weight, and the degradation kinetics were analyzed and compared with the corresponding parameters under ultrasonic and enzymolysis treatments. The degradation rate improved greatly and the time required to stabilize the rate was shortened compared with ultrasonic treatment, for example, more than 120 min was needed at 4 W/mL for ultrasonic treatment before stabilization with the degradation rate of 77.41%, whereas 80 min was needed for sonoenzymolysis treatment with the degradation rate of 91.44%. A lower molecular weight limit was established (7.15 × 104 Da at 4 W/mL for sonoenzymolysis treatment compared with 19.61 × 104 Da at 4 W/mL for ultrasonic treatment), with decreased time to approach the new limiting molecular weight (80 min compared with more than 120 min). The mass fraction of 104-105 Da fragment increased (61.02% at 4 W/mL for sonoenzymolysis treatment compared with 42.98% at 4 W/mL for ultrasonic treatment) and the dextran degradation kinetics for sonoenzymolysis under lower ultrasonic intensity fitted the Malhotra model well. Sonoenzymolysis treatment at the ultrasonic intensity of 4 W/mL for 80 min resulted in more 104-105 Da fragments in a shorter time. The results indicated that sonoenzymolysis can be applied as an efficient method to obtain clinical dextran.One of the most promising pharmaceutical research areas is developing advanced delivery systems for controlled and sustained drug release. The drug delivery system (DDS) can be designed to strengthen the pharmacological and therapeutic characteristics of different medicines. Natural polymers have resolved numerous commencing hurdles, which hindered the clinical implementation of traditional DDS. The naturally derived polymers furnish various advantages such as biodegradability, biocompatibility, inexpensiveness, easy availability, and biologically identifiable moieties, which endorse cellular activity in contrast to synthetic polymers. Among them, chitosan has recently been in the spotlight for devising safe and efficient DDSs due to its superior properties such as minimal toxicity, bio-adhesion, stability, biodegradability, and biocompatibility. The primary amino group in chitosan shows exceptional qualities such as the rate of drug release, anti-microbial properties, the ability to cross-link with various polymers, and macrophage activation. This review intends to provide a glimpse into different practical utilization of chitosan as a drug carrier. The first segment of the review will give cognizance into the source of extraction and chitosan’s remarkable properties. Further, we have endeavored to provide recent literature pertaining to chitosan applications in various drug delivery systems via different administration routes along with current patented chitosan formulations.Some members of genus Macrobrachium are important economically prawns and valuable objects for studying the innate immune defense mechanism of crustaceans. Studies have focused on immune responses against bacterial and fungal infections and have expanded to include antiviral immunity over the past two decades. Similar to all living organisms, prawns are exposed to viruses, including white spot syndrome virus, Macrobrachium rosenbergii nodavirus, and Decapod iridescent virus 1 and develop effective defense mechanisms. Here, we review current understanding of the antiviral host defense in two species of Macrobrachium. The main antiviral defense of Macrobrachium is the activation of intracellular signaling cascades, leading to the activation of cellular responses (apoptosis) and humoral responses (immune-related signaling pathways, antimicrobial and antiviral peptides, lectins, and prophenoloxidase-activating system).