Five impact factors including age, gender, clusters of multiple chronic conditions, social support, and ethnicity and culture were associated with people’s coping. Future healthcare plans should address the physical and psychological needs of people with multiple chronic conditions and highlight the importance of modifying their illness perceptions and enhancing appropriate coping strategies. © 2020 John Wiley & Sons Australia, Ltd.OBJECTIVE The aim of this study was to determine how the removal of non-impacted third molars (N-M3s) affects the periodontal status of neighboring second molars (M2s). SUBJECTS AND METHODS The periodontal condition of M2s for which the neighboring N-M3s were removed (more than 6 months previously) and those with intact N-M3s was analyzed in a cross-sectional observation study. In an additional case series, periodontal changes in M2s in response to adjacent N-M3 removal were observed during a 6-month follow-up period. RESULTS A total of 457 patients with 1,301 M2s were enrolled in this cross-sectional observational study. Compared to M2s with neighboring N-M3s, M2s without neighboring N-M3s (teeth removed more than 6 months previously) exhibited a 0.27-mm reduction in the average pocket depth (PD) (p  less then  .001) and a 0.38-fold reduced risk of at least one probing site with PD ≥5 mm (PD5+) (p  less then  .001). Subsequently, a 41-case follow-up study showed that 6 months after neighboring N-M3 extraction, the PD of the M2s decreased by 0.31 mm (p  less then  .001), while the incidence of PD5+ decreased by 21.9% when compared to the parameters detected before tooth extraction (p = .004). CONCLUSIONS Removing N-M3s was associated with an improved periodontal condition in neighboring M2s. © 2020 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. All rights reserved.The aim of this study was to investigate the possible influences of circPRKCI abnormal expression on lipopolysaccharide (LPS)-induced HK2 cell injury and its mechanism. The circPRKCI level was identified in serum samples from patients with urosepsis and healthy subjects, as well as LPS-treated HK2 cells by qRT-PCR. Cell viability, apoptosis, expression of proteins associated with apoptosis, and expression of pro-inflammatory cytokines in LPS-treated HK2 cells were measured. Effects of circPRKCI abnormal expression on LPS-induced HK2 cell injury were then evaluated. Afterward, the binding miRNA of circPRKCI and target gene of miRNA were identified, and the involvements of NF-kB pathway signaling pathway with the effects of circPRKCI were finally studied. CircPRKCI was significantly down-regulated in serum samples from patients with urosepsis and LPS-treated HK2 cells. LPS-induced decrease of cell viability, increase of cell apoptosis, as well as elevated productions of tumor necrosis factor (TNF)-α, interleukins (IL)-1β, IL-6, and IL-8 in HK2 cells were attenuated by overexpressed circPRKCI. In addition, circPRKCI negatively regulated the expression of miR-545, and miR-545 up-regulation reversed the inhibiting effects of circPRKCI overexpression on LPS-induced HK2 cell injury. Moreover, zinc finger E-box-binding homeobox 2 (ZEB2) was identified as a target gene of miR-545, and ZEB2 overexpression partly reversed the effects of miR-545 up-regulation on LPS-induced HK2 cell injury. Furthermore, NF-kB pathway was revealed to be associated to the effects of circPRKCI on LPS-induced HK2 cell injury. This research indicated that the highly expressed circPRKCI relieved inflammatory injury induced by LPS in HK2 cells by suppressing miR-545/ZEBs and depressing the briskness of NF-kB pathway. © 2020 International Union of Biochemistry and Molecular Biology.INTRODUCTION The United Kingdom and Ireland teachers of Human Disease/Clinical Medical Science for Dentistry (HD/CMSD) group continues to work together, and most recently collaborated to review current and future assessments. MATERIALS AND METHODS The first part of the review of assessments in HD/CMSD took place at a face-to-face meeting with presentations from delegates on assessments in their home institutions. The second, and larger part, comprised an online survey where all eighteen schools in the UK and Ireland participated. RESULTS All schools had some element of formative assessment, and the majority had a stand-alone summative assessment at the end of the HD/CMSD teaching block. Most schools had a written paper and practical elements to their assessments, most commonly a combination of a multiple-choice type question combined with an objective structured clinical examination (OSCE). There was a trend towards the use of single best answer (SBA) questions and a willingness among participants to share a question bank. All schools incorporated elements of HD/CMSD in their final examinations. DISCUSSION & CONCLUSION This collaboration promoted the sharing of developments in assessment for HD/CMSD and demonstrated a willingness to co-operate between institutions. Assessment in HD/CMSD in the UK and Ireland continues to be refined by those responsible for its content and delivery and assessment methods are progressing following evidence-based best practice. This article is protected by copyright. All rights reserved.BACKGROUND Hypergammaglobulinemic purpura of Waldenström (HGPW), a rare cutaneous eruption characterized by the triad of recurrent episodes of lower extremity petechiae, symptoms of stinging and burning, and lower extremity edema, is poorly described in children. Some children have been reported to follow a benign course, while others are eventually diagnosed with fulminant rheumatologic disease. OBJECTIVES To determine the distinguishing features of HGPW including the spectrum of disease manifestations and clinical outcomes. METHODS This is a multicenter, retrospective case series of six children with HGPW combined with a literature review of 45 previously published pediatric cases. RESULTS Most children were eventually diagnosed with systemic disease (63%) or developed autoantibody accumulation suggestive of evolving disease (71%). The most common diagnoses were Sjogren’s syndrome and systemic lupus erythematosus. The mean duration between onset of cutaneous eruption and diagnosis of systemic disease was 5.6 years, underscoring that HPGW patients often present with a rash that precedes the development of systemic symptoms. CONCLUSIONS Diagnosis of HGPW should prompt initial screening for rheumatologic disease with long-term rheumatology follow-up, as the majority of patients present with evolving manifestations of systemic disease. © 2020 Wiley Periodicals, Inc.A new bis-triarylborane tetracation (4-Ar2B-3,5-Me2C6H2)-C≡C-C≡C-(3,5-Me2C6H2-4-BAr2 [Ar = (2,6-Me2-4-NMe3-C6H2)+] (24+) shows distinctly different behaviour in its fluorimetric response than that of our recently published bis-triarylborane 5- (4-Ar2B-3,5-Me2C6H2)-2,2′-(C4H2S)2-5′-(3,5-Me2C6H2-4-BAr2) (34+). Single-crystal X-ray diffraction data on the neutral bis-triarylborane precursor 2N confirm its rod-like dumbbell structure, which is shown to be important for DNA/RNA targeting and also for BSA protein binding. Fluorimetric titrations with DNA/RNA/BSA revealed the very strong affinity of 24+ and indicated the importance of the properties of the linker connecting the two triarylboranes. Using the butadiyne- rather than a bithiophene linker resulted in an opposite emission effect (quenching vs enhancement), and 24+ bound to BSA 100 times stronger than 34+. Moreover, 24+ interacted strongly with ss-RNA, and circular dichroism (CD) results suggest ss-RNA chain-wrapping around the rod-like bis-triarylborane dumbbell structure like a thread around a spindle, a very unusual mode of binding of ss-RNA with small molecules. Furthermore, 24+ yielded strong Raman/SERS signals, allowing DNA or protein detection at ca. 10 nM concentrations. The above observations, combined with low cytotoxicity, efficient human cell uptake and organelle-selective accumulation make such compounds intriguing novel lead structures for bio-oriented, dual fluorescence/Raman-based applications. © 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.NEW FINDINGS Advanced heart failure patients who are implanted with left ventricular assist devices (LVADs) have a unique circulation that is characterised by a reduced or even absent arterial pulse. The remarkable survival of these patients is accompanied by circulatory complications, including stroke, gastro-intestinal bleeding and right-heart failure. Understanding the mechanisms related to the complications in LVAD patients will help the patients and also advance our fundamental understanding of the human circulation in general. ABSTRACT Some humans with chronic, advanced heart failure are surgically implanted with a left ventricular assist device (LVAD). Because the LVAD produces a continuous flow, a pulse is often absent in these patients. This allows for a unique investigation of the human circulation and a controversy around the 'need’ for a pulse. This medical debate also generates a more generic, fundamental discussion into what is 'normal’ arterial physiology & health. The comprehensive study and understanding of the arterial responses to drastically altered haemodynamics due to CF-LVADs, at rest and during activity, presents an opportunity to significantly increase our current understanding of the fundamental components of arterial regulation (flow, blood pressure, sympathetic activity, endothelial function, pulsatility) in a way that could never have been studied previously. In a series of four articles, we summarise the talks presented at the symposium entitled „Bionic Women and Men – Physiology lessons from implantable cardiac devices” during the 2019 Annual Meeting of The Physiological Society in Aberdeen, Scotland. The articles highlight the novel questions generated by physiological phenomena observed in LVAD patients and proposes future areas of interest within the field of cardiovascular physiology. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.The preparation of MoS 2 -polymer carbon nanodot (MoS 2 -PCND) hybrid material was accomplished by employing an easy and fast bottom-up synthetic approach. Specifically, MoS 2 -PCND was realized by the thermal decomposition of ammonium tetrathiomolybdate and the in-situ complexation of Mo with carboxylic acid units present in the surface of PCNDs. The newly prepared hybrid material was comprehensively characterized by spectroscopic, thermal and electron microscopy imaging means. The electrocatalytic activity of MoS 2 -PCND was examined against the hydrogen evolution reaction (HER) and compared with that attributed to the hybrid material prepared by a top-down approach, namely with MoS 2 -PCND(exf-fun), in which MoS 2 was firstly exfoliated and then covalently functionalized with PCNDs. The MoS 2 -PCND hybrid material showed superior electrocatalytic activity against HER with low Tafel slope value and excellent electrocatalytic stability, with an onset potential at -0.16 V vs RHE. The superior catalytic performance of MoS 2 -PCND was rationalized by considering the catalytic active sites of MoS 2 , the effective charge/energy-transfer phenomena from PCNDs to MoS 2 and the synergetic effect between MoS 2 and PCNDs within the hybrid material. © 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.