And upon As(V) and DMA exposures, antioxidant responses were more intense than that upon As(III) exposure. Further the results indicated that the antioxidant responses in lettuce were associated with the conversion and transport of As species.TiO2-nanoparticles (TiO2-NP) have the potential to impair plant development. Nevertheless, the metabolic processes behind the physiological responses to TiO2-NP are still far from being fully understood. In this study, Triticum aestivum plants were exposed for 21 days to different concentrations (0; 5; 50; 150 mg L-1) of TiO2-NP (P25). After treatment, the metabolite profiles of roots and leaves were analysed. The content of >70 % of the identified metabolites changed in response to P25 and the impact on metabolic pathways increased with TiO2-NP dose, with leaves showing higher alterations. Roots up-regulated monosaccharides, azelaic acid, and γ-aminobutanoic acid and triggered the tyrosine metabolism, whereas leaves up-regulated the metabolisms of reserve sugars and tocopherol, and the phenylalanine and tryptophan pathways. Both organs (mainly leaves) up-regulated the aspartate family pathway together with serine, alanine and valine metabolisms and the glycerolipids’ biosynthesis. In addition, the citrate and glyoxylate metabolisms were down-regulated in both organs (highest dose). Sugar biosynthesis breakdown, due to photosynthetic disturbances, shifted the cell metabolism to use amino acids as an alternative energy source, and both ROS and sugars worked as signalling molecules activating organ dependent antioxidant responses. Concluding, these NP-pollutants severely impact multiple crop metabolic pathways and may ultimately compromise plant performance.Nitroaromatic compounds (NACs) in the environment can cause serious public health and environmental problems due to their potential toxicity. This study established quantitative structure-toxicity relationship (QSTR) models for the acute oral toxicity of NACs towards rats following the stringent OECD principles for QSTR modelling. All models were assessed by various internationally accepted validation metrics and the OECD criteria. The best QSTR model contains seven simple and interpretable 2D descriptors with defined physicochemical meaning. Mechanistic interpretation indicated that van der Waals surface area, presence of C-F at topological distance 6, heteroatom content and frequency of C-N at topological distance 9 are main factors responsible for the toxicity of NACs. This proposed model was successfully applied to a true external set (295 compounds), and prediction reliability was analysed and discussed. Moreover, the rat-mouse and mouse-rat interspecies quantitative toxicity-toxicity relationship (iQTTR) models were also constructed, validated and employed in toxicity prediction for true external sets consisting of 67 and 265 compounds, respectively. These models showed good external predictivity that can be used to rapidly predict the rat oral acute toxicity of new or untested NACs falling within the applicability domain of the models, thus being beneficial in environmental risk assessment and regulatory purposes.Acid mine drainage (AMD) formation and toxic arsenic (As) contamination are serious environmental problems encountered worldwide. In this study, we investigated the crucial roles played by common secondary mineral phases formed during the natural weathering of pyrite-bearing wastes-soluble salts (melanterite, FeSO4·7H2O) and metal oxides (hematite, Fe2O3)-on AMD formation and As mobility under acidic conditions (pH 1.5-4) prevalent in historic tailings storage facilities, pyrite-bearing rock dumps and AMD-contaminated soils and sediments. Our results using a pyrite-rich natural geologic material containing arsenopyrite (FeAsS) showed that melanterite and hematite both directly-by supplying H+ and/or oxidants (Fe3+)-and indirectly-via changes in the nature of oxidation layer formed on pyrite-influenced pyrite oxidation dynamics. Based on SEM-EDS, DRIFT spectroscopy and XPS results, the oxidation layer on pyrite was mainly composed of ferric arsenate and K-Jarosite when melanterite was abundant with/without hematite but changed to Fe-oxyhydroxide/oxide and scorodite when melanterite was low and hematite was present. This study also observed the formation of a mechanically 'strong’ coating on pyrite that suppressed the mineral’s oxidation. Finally, As mobility under acidic conditions was limited by its precipitation as ferric arsenate, scorodite, or a Fe/Al arsenate phase, including its strong adsorption to Fe-oxyhydroxides/oxides.To identify the mechanisms relevant for the therapeutic effect of glatiramer acetate (GA), we studied T- and B- regulatory cells as well as GM-CSF expression in mice recovered from experimental autoimmune encephalomyelitis (EAE). Selective depletion of Tregs reduced but did not eliminate the ability of GA to ameliorate EAE, indicating a role for additional immune-subsets. The prevalence of Bregs in the periphery and the CNS of EAE-mice increased following GA-treatment. Furthermore, GA downregulated the pathological expression of GM-CSF, on both the protein and mRNA levels. Selleckchem 12-O-Tetradecanoylphorbol-13-acetate These findings corroborate the broad immunomodulatory mechanism of action of GA in EAE/MS.Background Several studies have indicated that mild systemic inflammation is associated with the risk of cognitive impairment. However, not every cognitive domain has been evaluated to have a correlation with peripheral inflammation in healthy individuals. Therefore, we aimed to investigate the association between C-reactive protein (CRP) as a marker of peripheral inflammation with various domains of cognition in healthy adults. Method This study consisted of 216 healthy native German adults (138 males and 78 females, mean age 39.12 ± 20.19 years) from „Leipzig Study for Mind-Body-Emotion Interactions” (LEMON) database. After the initial assessment and conducting the cognitive battery, a blood sample was collected and CRP level was evaluated. Patients were categorized into three groups based on their CRP level. Subsequently, demographic and cognitive features were compared across three groups and to confirm the association between CRP level and cognitive performance, general linear models (GLM) were applied. Results All California Verbal Learning Task (CVLT)-evaluated aspects of memory performance were inversely associated with CRP level, some of which remained significant after the adjustment for age, gender, education, smoking status and body mass index. Moreover, GLM analysis indicated that mean reaction time of the Test of Attentional Performance-Alertness (TAP-A) test (with and without signal) was also significantly associated with CRP level. Conclusion The current study indicated that healthy subjects with higher levels of CRP exhibit poorer performance in verbal learning memory and general wakefulness domains of cognition.Objective To determine the area of a safety window that excludes the vertebral artery for the safe access of the occipital condyle screws during occipitocervical fixation. link2 Methods This study included 138 cervical computed tomography angiograms. Six measurements per side were made in each imaging study. These measurements are from the vertebral artery to (A) the mastoid process, (B) the mastoid incisura, (C) the posterior condylar fossa, (D) the occipital condyle in its midline, and (E) the medial border of the condyle. We also measured from the tip of the mastoid process to the lower border of the occipital condyle on its lateral side (F). Results A total of 276 areas from 138 individuals were included, of which 51.4 % were men. The mean age was 54.2 ± 18.63 years. The mean variable measurements (mm) for all the population were 21 ± 4, 16 ± 3, 6 ± 2, 3 ± 2, 2 ± 1 and 35 ± 4 for variables A-F, respectively. We found significant differences between sex when we compared measurements A (p = 0.003), C (p = 0.001), D (p = 0.000) and F (p = 0.000). The incidence rate of dominance for the vertebral artery was 18.8 % and 30.4 % for right and left respectively. Conclusion Women had significantly smaller measures than men. This could indicate a higher risk of iatrogenic injury secondary to a smaller vertebral artery-free area. Results may guide surgeons in the pre-surgical planning aiming to reduce the risk of iatrogenic injuries to the vertebral artery.The poultry red mite, Dermanyssus gallinae, poses a significant threat to hen health and poultry husbandry. D. gallinae has typically been controlled using synthetic acaricides, like pyrethroids, but increased resistance to pyrethroids has been found in poultry red mite populations worldwide. Pyrethroids resistance in arthropods has been associated to cytochrome P450 monooxygenases (P450s), a main member of a group of detoxification enzymes. To explore the potential contribution of P450s to the resistance to pyrethroids in D. gallinae, we first identified and then characterized four P450s genes. Phylogenetic analysis revealed that the four P450s genes in D. gallinae belong to three different clades, with two in the CYP-6, one in the CYP-4 and one in the CYP-2. All four P450s genes were expressed in a similar pattern in D. gallinae at different stages of development, and showed high expression in the adult stage, indicating that they played a role in mite development. Simultaneously, constitutive over-expression of Deg-CYP-3, a clade associated with pesticide metabolism, was detected in a resistant strain (RS) compared with a susceptible strain (SS). When exposed to beta-cypermethrin, the four P450s gene transcripts in the RS strain increased in a time-dependent manner. In particular, Deg-CYP-3 expression increased 5-fold compared to gene expression in control group at 12 h, although the four P450s genes were not induced in the SS strain. Our results show the first insights into the molecular characteristics of P450s genes in D. link3 gallinae. The elevated presence of P450s genes in the RS strain, indicated by their constitutive over-expression and their inducible expression, suggests that they confer resistance to beta-cypermethrin, and are involved in its detoxification.The present study involved identification of genes which are present in the genome of native bacteria to make them effective tools for bioremediation of persistent organic pollutants (POPs). During this study, forty-one POPs (naphthalene, toluene and petrol) metabolizing bacteria were isolated from tannery effluents and petrol contaminated soil samples by successive enrichment culturing. The taxonomic diversity and gene repertoire conferring POPs degradation ability to the isolated bacterial community were studied through whole genome shotgun sequencing of DNA consortium. The DNA consortium contained equimolar concentration of DNA extracted from each bacterial isolate using organic method. To add a double layer of confirmation the established DNA consortium was subjected to 16S rRNA metagenome sequencing and whole genome shotgun sequencing analysis. Biodiversity analysis revealed that the consortium was composed of phyla Firmicutes (80 %), Proteobacteria (12 %) and Actinobacteria (5%). Genera found included Bacillus (45 %), Burkholderia (25 %), Brevibacillus (9%) and Geobacillus (4%). Functional profiling of consortium helped us to identify genes associated with degradation pathways of a variety of organic compounds including toluene, naphthalene, caprolactam, benzoate, aminobenzoate, xylene, 4-hydroxyphenyl acetic acid, biphenyl, anthracene, aminobenzoate, chlorocyclohexane, chlorobenzene, n-phenylalkanoic acid, phenylpropanoid, salicylate, gentisate, central meta cleavage of aromatic compounds, cinnamic acid, catechol and procatechuate branch of β-ketoadipate pathway, phenyl-acetyl CoA and homogentisate catabolic pathway. The information thus generated has ensured not only biodegradation potential but also revealed many possible future applications of the isolated bacteria.